Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)

Overview

  • Product nameAnti-Synapsin I (phospho S9) antibody [EP2162Y]
    See all Synapsin I primary antibodies
  • Description
    Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9)
  • Tested applicationsSuitable for: WB, IHC-FoFrmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Synapsin I (phospho S9). The exact sequence is proprietary.

  • Positive control
    • WB: Human, mouse and rat brain tissue lysates, untreated or treated with AP.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-FoFr Use at an assay dependent concentration. PubMed: 25232103
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionNeuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
    • Involvement in diseaseDefects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.
    • Sequence similaritiesBelongs to the synapsin family.
    • Post-translational
      modifications
      Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCell junction > synapse. Golgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Brain protein 4.1 antibody
      • SYN 1 antibody
      • SYN 1a antibody
      • SYN 1b antibody
      • SYN I antibody
      • SYN1 antibody
      • SYN1_HUMAN antibody
      • SYN1a antibody
      • SYN1b antibody
      • Synapsin 1 antibody
      • Synapsin I antibody
      • Synapsin-1 antibody
      • Synapsin1 antibody
      • SynapsinI antibody
      • SYNI antibody
      see all

    Anti-Synapsin I (phospho S9) antibody [EP2162Y] images

    • All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/1000 dilution

      Lane 1 : Human brain whole cell lysate - untreated
      Lane 2 : Human brain whole cell lysate - treated with Alkaline Phosphatase
      Lane 3 : Human brain whole cell lysate - untreated
      Lane 4 : Human brain whole cell lysate - treated with Alkaline Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 74 kDa
      Observed band size : 77 kDa (why is the actual band size different from the predicted?)

      Exposure time:

      Lanes 1 and 2: 1 second.

      Lanes 3 and 4: 15 seconds.

      Blocking and dilution buffer:

      Lanes 1 and 2: 5% NFDM/TBST.

      Lanes 3 and 4: 2% BSA/TBST.

    • All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/5000 dilution

      Lane 1 : Human brain whole cell lysate - untreated
      Lane 2 : Human brain whole cell lysate - treated with Alkaline Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 74 kDa
      Observed band size : 77 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/200 dilution

      Lane 1 : Mouse brain whole cell lysate - untreated
      Lane 2 : Mouse brain whole cell lysate - treated with Alkaline Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 74 kDa
      Observed band size : 77 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/1000 dilution

      Lane 1 : Rat brain whole cell lysate - untreated
      Lane 2 : Rat brain whole cell lysate - treated with Alkaline Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 74 kDa
      Observed band size : 77 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 second

      Blocking and dilution buffer: 2% BSA/TBST.

    • All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)

      Lane 1 : human brain tissue lysates, untreated
      Lane 2 : human brain tissue lysates, treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 74 kDa
      Observed band size : 74 kDa
    • Dot blot analysis of Synapsin I (pS9) phospho peptide (lane 1) and Synapsin I non-phospho peptide (lane 2) labelling Synapsin I (pS9) with ab76260 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    References for Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)

    This product has been referenced in:
    • Campos CA & Ritter RC NMDA-type glutamate receptors participate in reduction of food intake following hindbrain melanocortin receptor activation. Am J Physiol Regul Integr Comp Physiol 308:R1-9 (2015). Read more (PubMed: 25394828) »
    • Campos CA  et al. Central vagal afferent endings mediate reduction of food intake by melanocortin-3/4 receptor agonist. J Neurosci 34:12636-45 (2014). IHC-FoFr ; Rat . Read more (PubMed: 25232103) »

    See all 2 Publications for this product

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