The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Detects a band of approximately 74, 54 kDa (predicted molecular weight: 57.5 kDa).
Use at an assay dependent dilution. PubMed: 19714568
Synapsin II is a member of the synapsin gene family. Synapsins are neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family is a neuron-specific phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal.
Western blot - Anti-Synapsin II antibody (ab13258)Image from Ramos C et al., J Biol Chem. 2012 Jun 8;287(24):20176-86. doi: 10.1074/jbc.M112.347468. Epub 2012 Apr 23. Fig 5.; June 8, 2012 The Journal of Biological Chemistry, 287, 20176-20186.
Predicted band size : 57.5 kDa
Image from Ramos C et al., J Biol Chem. 2012 Jun 8;287(24):20176-86. doi: 10.1074/jbc.M112.347468. Epub 2012 Apr 23. Fig 5.; June 8, 2012 The Journal of Biological Chemistry, 287, 20176-20186.
Western blot analysis of rat cerebellum tissue, staining Synapsin II with ab13258.
Samples were immunoprecipitated with antibodies against CB or mGluR4 and co-immunoprecipitated Synapsin II was detected using ab13258. Synapsin I was detected using ab8 - Rabbit polyclonal to Synapsin I.
References for Anti-Synapsin II antibody (ab13258)
This product has been referenced in:
Shi W et al. Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury. Acta Biomater45:247-261 (2016).
Read more (PubMed: 27592818) »
Ramos C et al. Native presynaptic metabotropic glutamate receptor 4 (mGluR4) interacts with exocytosis proteins in rat cerebellum. J Biol Chem287:20176-86 (2012).
Read more (PubMed: 22528491) »