Overview

  • Product name
    Anti-Synapsin III antibody
    See all Synapsin III primary antibodies
  • Description
    Rabbit polyclonal to Synapsin III
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Synapsin III aa 370-399 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O14994

  • Positive control
    • Human testis tissue; NCI H460 cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab175533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 63 kDa.

Target

  • Function
    May be involved in the regulation of neurotransmitter release and synaptogenesis.
  • Tissue specificity
    Neuron specific. Detected predominantly in brain.
  • Sequence similarities
    Belongs to the synapsin family.
  • Cellular localization
    Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Peripheral membrane protein localized to the cytoplasmic surface of synaptic vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • cN28H9.2 (synapsin III) antibody
    • OTTHUMP00000028987 antibody
    • SYN 3 antibody
    • SYN3 antibody
    • SYN3_HUMAN antibody
    • Synapsin 3 antibody
    • Synapsin III antibody
    • Synapsin-3 antibody
    see all

Images

  • Immunohistochemical analysis of  formalin fixed, paraffin embedded Human testis tissue labeling Synapsin III with ab175533 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-Synapsin III antibody (ab175533) at 1/1000 dilution + NCI H460 cell line lysate at 15 µg
    Developed using the ECL technique

    Predicted band size : 63 kDa

References

ab175533 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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