Anti-Synaptobrevin antibody [SP11] (ab11104)

Overview

  • Product nameAnti-Synaptobrevin antibody [SP11]
    See all Synaptobrevin primary antibodies
  • Description
    Mouse monoclonal [SP11] to Synaptobrevin
  • Specificityab11104 recognises the human synaptic vesicle protein synaptobrevin, VAMP; it is not known whether it recognises VAMP1 or VAMP2. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Crude human synaptic immunoprecipitate.

  • Positive control
    • human brain lysate
  • General notesThis product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 13 kDa.
IHC-P Use at an assay dependent concentration. Do not perform antigen retrieval.
ELISA Use at an assay dependent concentration.

Target

  • RelevanceSynaptobrevins are highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Synaptobrevins, Syntaxins, and the 25 kDa synaptosomal associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane.
  • Cellular localizationCytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane. Single-pass type IV membrane protein. Cell junction > synapse > synaptosome. Isoform 3 is found in mitochondria, mitochondrial outer membrane.
  • Database links
  • Alternative names
    • SYB antibody
    • SYB1 antibody
    • Synaptobrevin 1 antibody
    • VAMP antibody
    • VAMP1 antibody
    • Vesicle associated membrane protein 1 antibody
    see all

Anti-Synaptobrevin antibody [SP11] images

References for Anti-Synaptobrevin antibody [SP11] (ab11104)

ab11104 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. I am sorry to hear that you are having problems with the 3 antibodies ab53723, ab11104 and ab3265. Unfortunately, ab53723 has not been tested for IHC-Frozen, horse and pig samples. Also, ab11104 has not yet been teste...

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I'm afraid we have been unsuccessful at finding references detailing which isoform is detected by ab11104 clone SP11, it appears no one has reported which isoform in particular is detected and as the immunogen is crude human synaptic immunoprecipitate ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"