The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 25 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionInvolved in the positive regulation of dopamine transporter activity. Probably facilitates the physical and functional interactions of the transporter with the dopamine vesicular storage system, allowing a more efficient loading of the vesicles with extracellular dopamine after release.
Tissue specificityExpressed in brain and placenta.
Sequence similaritiesBelongs to the synaptogyrin family. Contains 1 MARVEL domain.
Cellular localizationMembrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle. Cell junction > synapse. Found at the neuromuscular synapses.