Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/128000.
    WB: Use at a concentration of 0.01 - 01 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa). A minor band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunising peptide. Can be blocked with Synaptogyrin 4 peptide (2-14) (ab45658).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • Anti-Synaptogyrin 4 antibody (ab27480) at 0.01 µg/ml + Human brain lysate (35µg protein in RIPA buffer).

      Predicted band size : 26 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.

    References

    ab27480 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab27480.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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