Overview

  • Product name
    Anti-Synaptojanin antibody
    See all Synaptojanin primary antibodies
  • Description
    Rabbit polyclonal to Synaptojanin
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within: GVARREMEAP KSPGTTRKDN IGRSQPSPQA GLAGPGPAGY STARPTIPPR, internal sequence amino acids 1152-1201 of Human Synaptojanin (NP_003886)

  • Positive control
    • Human fetal stomach lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 173 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Inositol 5-phosphatase which has a role in clathrin-mediated endocytosis.
  • Tissue specificity
    Concentrated at clathrin-coated endocytic intermediates in nerve terminals. Isoform 1 is more enriched than isoform 2 in developing brain as well as non-neuronal cells. Isoform 2 is very abundant in nerve terminals.
  • Sequence similarities
    Belongs to the synaptojanin family.
    In the central section; belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SAC domain.
  • Domain
    Binds to EPS15 (a clathrin coat-associated protein) via a C-terminal domain containing three Asn-Pro-Phe (NPF) repeats.
    The C-terminal proline-rich region mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4 antibody
    • 5-trisphosphate 5-phosphatase 1 antibody
    • Inositol 5' phosphatase (synaptojanin 1) antibody
    • Inositol 5' phosphatase antibody
    • INPP 5G antibody
    • INPP5G antibody
    • KIAA0910 antibody
    • PARK20 antibody
    • Polyphosphoinositide phosphatase antibody
    • Synaptic inositol 1 4 5 trisphosphate 5 phosphatase 1 antibody
    • Synaptic inositol 145 trisphosphate 5 phosphatase 1 antibody
    • Synaptic inositol-1 antibody
    • Synaptojanin 1 antibody
    • Synaptojanin 1 polyphosphoinositide phosphatase antibody
    • Synaptojanin-1 antibody
    • Synaptojanin1 antibody
    • Synj 1 antibody
    • Synj1 antibody
    • SYNJ1_HUMAN antibody
    see all

Images

  • Anti-Synaptojanin antibody (ab94912) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal stomach lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 173 kDa

References

ab94912 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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