Overview

  • Product nameAnti-Synaptojanin antibody [AC1]
    See all Synaptojanin primary antibodies
  • Description
    Mouse monoclonal [AC1] to Synaptojanin
  • Specificityab24591 is specific for rat synsptojanin 1 protein. It may cross-react only very weakly with human synaptojanin 1. ab24591 does not cross react with mouse.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Rat
    Does not react with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to C terminal amino acids 1156-1286 of Rat Synaptojanin

  • General notes


    The C-terminal proline-rich region of Synaptojanin 1 mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesThe C-terminal proline-rich region of Synaptojanin 1 mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2.
  • ClonalityMonoclonal
  • Clone numberAC1
  • Isotypeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/20 - 1/100.
WB 1/100. Detects a band of approximately 145-170 kDa.
IHC-Fr 1/20.
IP Use at an assay dependent dilution.

Target

  • FunctionInositol 5-phosphatase which has a role in clathrin-mediated endocytosis.
  • Tissue specificityConcentrated at clathrin-coated endocytic intermediates in nerve terminals. Isoform 1 is more enriched than isoform 2 in developing brain as well as non-neuronal cells. Isoform 2 is very abundant in nerve terminals.
  • Sequence similaritiesBelongs to the synaptojanin family.
    In the central section; belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SAC domain.
  • DomainBinds to EPS15 (a clathrin coat-associated protein) via a C-terminal domain containing three Asn-Pro-Phe (NPF) repeats.
    The C-terminal proline-rich region mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4 antibody
    • 5-trisphosphate 5-phosphatase 1 antibody
    • Inositol 5' phosphatase (synaptojanin 1) antibody
    • Inositol 5' phosphatase antibody
    • INPP 5G antibody
    • INPP5G antibody
    • KIAA0910 antibody
    • PARK20 antibody
    • Polyphosphoinositide phosphatase antibody
    • Synaptic inositol 1 4 5 trisphosphate 5 phosphatase 1 antibody
    • Synaptic inositol 145 trisphosphate 5 phosphatase 1 antibody
    • Synaptic inositol-1 antibody
    • Synaptojanin 1 antibody
    • Synaptojanin 1 polyphosphoinositide phosphatase antibody
    • Synaptojanin-1 antibody
    • Synaptojanin1 antibody
    • Synj 1 antibody
    • Synj1 antibody
    • SYNJ1_HUMAN antibody
    see all

Anti-Synaptojanin antibody [AC1] images

  • Immunofluorescence staining using Mouse monoclonal [AC1] to Synaptojanin - Synaptic Marker (ab24591; 30 µg/ml ) on rat Hippocampal neuron culture (paraformadlehyde fixed - see review).  Confocal image showing blue nuclear counterstain.

References for Anti-Synaptojanin antibody [AC1] (ab24591)

This product has been referenced in:
  • Latimer CS  et al. Vitamin D prevents cognitive decline and enhances hippocampal synaptic function in aging rats. Proc Natl Acad Sci U S A 111:E4359-66 (2014). Rat . Read more (PubMed: 25267625) »
  • Nemoto Y  et al. Identification and characterization of a synaptojanin 2 splice isoform predominantly expressed in nerve terminals. J Biol Chem 276:41133-42 (2001). Read more (PubMed: 11498538) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Hippocampal neuron culture)
Specification Hippocampal neuron culture
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5%
Username

Dr. Randal Moldrich

Verified customer

Submitted May 30 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"