Overview

  • Product nameAnti-Synaptophysin antibody [4E206]
    See all Synaptophysin primary antibodies
  • Description
    Mouse monoclonal [4E206] to Synaptophysin
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synaptic vesicle-containing fractions immunoprecipitated from human brain homogenates using anti-human Synaptophysin monoclonal antibodies.

  • Positive control
    • Mouse and rat brain tissue extract. No reactivity was detected in mouse liver extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab18008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 38 kDa.
IP 1/100.
IHC-P Use at an assay dependent dilution.

Target

  • FunctionPossibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • Tissue specificityCharacteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • Involvement in diseaseMental retardation, X-linked, SYP-related
  • Sequence similaritiesBelongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • DomainThe calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • Post-translational
    modifications
    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localizationCytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

References for Anti-Synaptophysin antibody [4E206] (ab18008)

This product has been referenced in:
  • Lee J  et al. Conditional deletion of the focal adhesion kinase FAK alters remodeling of the blood-brain barrier in glioma. Cancer Res 70:10131-40 (2010). Read more (PubMed: 21159635) »
  • Winden KD  et al. The organization of the transcriptional network in specific neuronal classes. Mol Syst Biol 5:291 (2009). WB ; Mouse . Read more (PubMed: 19638972) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (Cortex)
Loading amount 50 µg
Specification Cortex
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5%
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Submitted Jan 15 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"