Our Abpromise guarantee covers the use of ab14692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).|
|IHC-FoFr||1/500. PubMed: 24846136|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||1/1000. PubMed: 19096824|
This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
Synaptophysin antibody (ab14692) western blot on mouse forebrain extract (10µg protein/lane). The blot was exposed for 10 minutes to autoradiographic film. Ladder markers from 7-194 kDa.
Lane 1 = 1/500 ab14692
Lane 2 = 1/200 ab14692
ab14692 staining Synaptophyson in mouse retina (8 weeks old) and brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). 10µm frozen sections were fixed with 4% paraformaldehyde for 10 minutes and blocked with 10% donkey serum for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS) overnight at 4°C. An Alexa Fluor 488®-conjugated donkey anti-rabbit IgG polyclonal (1/1000 in PBS) was used as the secondary antibody (1 hour at room temperature). Counterstained with Hoechst 33258 (blue) for 10 minutes at room temperature.
Images shows: GCL - ganglion cell layer, IPL - inner plexiform layer, INL - inner nuclear layer, OPL - outer plexiform layer, ONL - outer nuclear layer; DG - dentate gyrus region of the hippocampus.
ab14692 staining cultured ED18 rat primary hippocampal neurons by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured cells were then stained with ab14692 at 1/200 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for overnight at 4°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. Synaptophysin expressed in axon, dendrites and particularly carrying in the vesicles.
Immunohistochemical analysis of formaldehyde-fixed, paraffin-embedded human CNS sections labelling Synaptophysin with ab14692 at a dilution of 1/1000. The secondary antibody used was biotin conjugated polyclonal goat vector at 1/250. Antigen retrieval was heat mediated using citrate.