Overview

  • Product name
    Anti-Synaptophysin antibody
    See all Synaptophysin primary antibodies
  • Description
    Rabbit polyclonal to Synaptophysin
  • Specificity
    The antibody does not cross-react other synaptic vesicle proteins such as synaptotagmin, GAP45, and SNAP25 protiens.
  • Tested applications
    Suitable for: WB, IP, IHC-FoFr, IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human Synaptophysin aa 41-62.
    Sequence:

    FATCGSYSGELQLSVDCANKTE

  • Positive control
    • WB: Mouse brain tissue lysate. IHC-P: Rat brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab14692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
IP 1/250.
IHC-FoFr 1/500. PubMed: 24846136
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200.
IHC-P 1/1000. PubMed: 19096824

Target

  • Function
    Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • Tissue specificity
    Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • Involvement in disease
    Mental retardation, X-linked, SYP-related
  • Sequence similarities
    Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • Domain
    The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • Post-translational
    modifications
    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localization
    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

Images

  • Immunohistochemical analysis of formaldehyde-fixed, paraffin-embedded human CNS sections labelling Synaptophysin with ab14692 at a dilution of 1/1000. The secondary antibody used was biotin conjugated polyclonal goat vector at 1/250. Antigen retrieval was heat mediated using citrate.

    See Abreview

  • ab14692 staining Synaptophyson in mouse retina (8 weeks old) and brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). 10µm frozen sections were fixed with 4% paraformaldehyde for 10 minutes and blocked with 10% donkey serum for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS) overnight at 4°C. An Alexa Fluor 488®-conjugated donkey anti-rabbit IgG polyclonal (1/1000 in PBS) was used as the secondary antibody (1 hour at room temperature). Counterstained with Hoechst 33258 (blue) for 10 minutes at room temperature.

    Images shows: GCL - ganglion cell layer, IPL - inner plexiform layer, INL - inner nuclear layer, OPL - outer plexiform layer, ONL - outer nuclear layer; DG - dentate gyrus region of the hippocampus.

    See Abreview



  • Predicted band size : 38 kDa

    This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom

    Synaptophysin antibody (ab14692) western blot on mouse forebrain extract (10µg protein/lane). The blot was exposed for 10 minutes to autoradiographic film. Ladder markers from 7-194 kDa.

    Lane 1 = 1/500 ab14692
    Lane 2 = 1/200 ab14692

  • ab14692 staining cultured ED18 rat primary hippocampal neurons by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured cells were then stained with ab14692 at 1/200 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for overnight at 4°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. Synaptophysin expressed in axon, dendrites and particularly carrying in the vesicles.

    See Abreview

References

This product has been referenced in:
  • Reim D  et al. Proteomic Analysis of Post-synaptic Density Fractions from Shank3 Mutant Mice Reveals Brain Region Specific Changes Relevant to Autism Spectrum Disorder. Front Mol Neurosci 10:26 (2017). Read more (PubMed: 28261056) »
  • Caviedes A  et al. Endothelial Nitric Oxide Synthase Is Present in Dendritic Spines of Neurons in Primary Cultures. Front Cell Neurosci 11:180 (2017). Read more (PubMed: 28725180) »

See all 30 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Permeabilization
Yes - 0.3% Triton X-100 included in blocking step
Specification
Brain
Blocking step
10% Donkey serum 3% BSA 0.3% Triton X-100 as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 20 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (CNS)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
CNS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Username

Jim Manavis

Verified customer

Submitted Aug 16 2016

Thank you for that confirmation.

I have now arranged for a replacement vial to be sent to you. This is on the order number xxxxx(Purchase order number xxxxxx). This should be with you on Wednesday. If you have any problems receiving it please...

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Thank you for providing that information.

From having reviewed the protocol details provided there are q few thinks I would suggest would be worthwhile to try to obtain the best staining with the antibody.

1. I would suggest increas...

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Thank you for providing that information. That has clarified the situation encountered by the customer very well. Could I ask for a few more details to be provided:

1. Which type of tissue samples are being stained? Human? Mouse? From which o...

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Thank you for your inquiry.

Please find the Certificate of Compliance for lot number GR94362 attached to this email.

Have a good day.

Thank you for contacting us.

I am sorry to hear one of your customers have been experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing c...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer,pH 6.0
Blocking step
Milk as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 04 2012

Thank you for contacting us.

Here please find the information about our testing discount program. Below I also include information about our Abpromise guarantee.

For UNTESTED species and/or applications, we have established a testi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Hippocampal neuron culture)
Specification
Hippocampal neuron culture
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 14 2012

1-10 of 16 Abreviews or Q&A

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