The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).
Use at an assay dependent concentration.
Use a concentration of 10 µg/ml.
FunctionPossibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
Tissue specificityCharacteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
Involvement in diseaseMental retardation, X-linked, SYP-related
Sequence similaritiesBelongs to the synaptophysin/synaptobrevin family. Contains 1 MARVEL domain.
DomainThe calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
Post-translational modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
ICC/IF image of ab53166 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53166 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Synaptophysin antibody (ab53166)
This product has been referenced in:
Arnold AS et al. Morphological and functional remodelling of the neuromuscular junction by skeletal muscle PGC-1a. Nat Commun5:3569 (2014).
Read more (PubMed: 24686533) »
Lo AC et al. Dose-dependent improvements in learning and memory deficits in APPPS1-21 transgenic mice treated with the orally active Aß toxicity inhibitor SEN1500. Neuropharmacology75C:458-466 (2013).
Read more (PubMed: 24035915) »