Overview

  • Product name
    Anti-Synaptophysin antibody
    See all Synaptophysin primary antibodies
  • Description
    Rabbit polyclonal to Synaptophysin
  • Specificity
    This antibody reacts with a hydrophilic peptide sequence of synaptophysin.
  • Tested applications
    Suitable for: IHC-FoFr, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab7837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • Tissue specificity
    Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • Involvement in disease
    Mental retardation, X-linked, SYP-related
  • Sequence similarities
    Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • Domain
    The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • Post-translational
    modifications
    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localization
    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

Anti-Synaptophysin antibody images

  • ICC/IF image of ab7837 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7837, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Synaptophysin antibody (ab7837)

This product has been referenced in:
  • McClean PL & Hölscher C Liraglutide can reverse memory impairment, synaptic loss and reduce plaque load in aged APP/PS1 mice, a model of Alzheimer's disease. Neuropharmacology 76 Pt A:57-67 (2014). Read more (PubMed: 23973293) »
  • Faivre E & Hölscher C D-Ala2GIP facilitated synaptic plasticity and reduces plaque load in aged wild type mice and in an Alzheimer's disease mouse model. J Alzheimers Dis 35:267-83 (2013). IHC ; Mouse . Read more (PubMed: 23568101) »

See all 3 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Vadivel Parthsarathy

Verified customer

Submitted Aug 10 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Vadivel Parthsarathy

Verified customer

Submitted Oct 15 2013

Thank you for contacting us.

Here please find the information about our testing discount program. Below I also include information about our Abpromise guarantee.

For UNTESTED species and/or applications, we have established a testi...

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Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IF. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in ret...

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This antibody has not yet been tested for cross reactivity with rat. However, it works well in IHC and is a polyclonal and will thus recognise a number of epitopes on the immunogen. So it is possible that it will cross react with rodent synaptophysin. ...

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The anitbody has not yet been tested for cross reativity with rat and mouse. However, it works well in IHC and is a polyclonal and will thus recognise a number of epitopes on the immunogen. I'd therefore predict that it will cross react with rodent syn...

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