Anti-Synaptophysin antibody [EP1098Y] (ab52636)


  • Product nameAnti-Synaptophysin antibody [EP1098Y]
    See all Synaptophysin primary antibodies
  • Description
    Rabbit monoclonal [EP1098Y] to Synaptophysin
  • Tested applicationsSuitable for: IHC-P, WB, IP, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on Synaptophysin (Human)

  • Positive control
    • Rat brain membrane. Human medullablastoma. PC12 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Synaptophysin antibody (HRP) [EP1098Y] (ab204260)



Our Abpromise guarantee covers the use of ab52636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
IP 1/50 - 1/100.
Flow Cyt 1/20 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/250 - 1/500.


  • FunctionPossibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • Tissue specificityCharacteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • Involvement in diseaseMental retardation, X-linked, SYP-related
  • Sequence similaritiesBelongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • DomainThe calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • Post-translational
    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localizationCytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

Anti-Synaptophysin antibody [EP1098Y] images

  • PC12 cells labelled with ab52636 at 1/250 - 1/500 dilution
  • Human medulloblastoma labelled with ab52636 at 1/250 - 1/500 dilution
  • Anti-Synaptophysin antibody [EP1098Y] (ab52636) at 1/200000 dilution + Rat brain membrane

    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size : 34 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing PC12 cells stained with ab52636 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52636, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-Synaptophysin antibody [EP1098Y] (ab52636)

This product has been referenced in:
  • Getz AM  et al. Tumor suppressor menin is required for subunit-specific nAChR a5 transcription and nAChR-dependent presynaptic facilitation in cultured mouse hippocampal neurons. Sci Rep 7:1768 (2017). WB ; Mouse . Read more (PubMed: 28496137) »
  • Curcio M  et al. Brain ischemia downregulates the neuroprotective GDNF-Ret signaling by a calpain-dependent mechanism in cultured hippocampal neurons. Cell Death Dis 6:e1645 (2015). Read more (PubMed: 25675305) »

See all 14 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse primary neurons, 2wks in culture)
Permeabilization Yes - 0.5% TX100
Specification mouse primary neurons, 2wks in culture
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Nov 04 2016

Application Western blot
Sample Mouse Cell lysate - whole cell (brain)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 25 µg
Specification brain
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Nov 04 2016

The immunogen is located in a cytoplasmic region of the protein.

I believe undifferentiated PC12 cells should give you good results as a positive control for ab52636 and ab32127. The cycle of the PC12 cells in these images is unknown however, the cells are grown to 90-95% confluent before they are harvested.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for contacting us last week.

I am sorry that you have been experiencing problems with ab52636. Below is a copy of the protocol used to characterize the antibody in IHC. The antibody has not been specifically tested on primary mouse...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (ht1080)
Loading amount 100000 cells
Specification ht1080
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted May 23 2012

Thank you for contacting us.

Here please find the information about our testing discount program. Below I also include information about our Abpromise guarantee.

For UNTESTED species and/or applications, we have established a testi...

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Thank you for contacting Abcam. My colleague is out of the office but we have recieved the information that you requested so I thought I would pass that on to you. The concentration of lot GR27991-1 of ab52636 Anti-Synaptophysin antibody is 0.76mg/mL. ...

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Thank you for getting back to me. As I have mentioned in the previous email, there are many reasons to the differences but the most common factor is because of the gel percentage, intensity of current, etc which will cause the protein to run a bit ...

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