Recombinant
RabMAb

Anti-Synaptophysin antibody [EPR1097-2] (ab178412)

Overview

  • Product name
    Anti-Synaptophysin antibody [EPR1097-2]
    See all Synaptophysin primary antibodies
  • Description
    Rabbit monoclonal [EPR1097-2] to Synaptophysin
  • Tested applications
    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Synaptophysin aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P08247

  • Positive control
    • Rat brain, PC12, Mouse brain and Human brain lysates; PC12 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab178412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
    • Tissue specificity
      Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
    • Involvement in disease
      Mental retardation, X-linked, SYP-related
    • Sequence similarities
      Belongs to the synaptophysin/synaptobrevin family.
      Contains 1 MARVEL domain.
    • Domain
      The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
    • Post-translational
      modifications
      Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
    • Cellular localization
      Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Major synaptic vesicle protein p38 antibody
      • MRX96 antibody
      • MRXSYP antibody
      • Syn p38 antibody
      • Synaptophysin antibody
      • Syp antibody
      • SYPH antibody
      • SYPH_HUMAN antibody
      • SypI antibody
      see all

    Images

    • Immunofluorescent analysis of PC12 cells labeling Synaptophysin with ab178412 at 1/50 (green) and DAPI staining (blue).

    • All lanes : Anti-Synaptophysin antibody [EPR1097-2] (ab178412) at 1/1000 dilution

      Lane 1 : Rat brain lysate
      Lane 2 : PC12 lysate
      Lane 3 : Mouse brain lysate
      Lane 4 : Human brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 33 kDa
      Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    References

    ab178412 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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