Overview

  • Product name
    Anti-Synaptophysin antibody [SP15]
    See all Synaptophysin primary antibodies
  • Description
    Mouse monoclonal [SP15] to Synaptophysin
  • Specificity
    Ab11105 recognises an antigen which appears to be identical to the human synaptic vesicle protein synaptophysin. It reacts with synaptophysin expressed in CHO cells (Honer WG et al. 1993); (Wakabayashi K et al. 1994).
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat, Hamster, Human, Pig
  • Immunogen

    Crude human synaptic immunoprecipitate.

  • Positive control
    • Brain.
  • General notes
    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Purified IgM
  • Purification notes
    Purified IgM prepared by gel filtration from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    SP15
  • Myeloma
    NS0
  • Isotype
    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000.
ELISA Use at an assay dependent dilution.
WB 1/500. Predicted molecular weight: 37.6 kDa.

Target

  • Function
    Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • Tissue specificity
    Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • Involvement in disease
    Mental retardation, X-linked, SYP-related
  • Sequence similarities
    Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • Domain
    The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • Post-translational
    modifications
    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localization
    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

Images

  • ab11105, at a dilution of 1/25, staining Synaptophysin in human pancreas section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

This product has been referenced in:
  • Ji YC  et al. Extraventricular neurocytoma in the left temporal lobe: A case report and review of the literature. Oncol Lett 11:3579-3582 (2016). Read more (PubMed: 27313678) »
  • Micheva KD & Smith SJ Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits. Neuron 55:25-36 (2007). IHC . Read more (PubMed: 17610815) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. Synaptophysin antibodies ab11105 and ab24729 are derived from two different monoclonal clones: [SP15] and [SP17]. They were raised against different immunogens and recognise different epitopes. Both antisera have als...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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