Anti-Synaptophysin antibody [YE269] (ab32127)

Overview

  • Product nameAnti-Synaptophysin antibody [YE269]
    See all Synaptophysin primary antibodies
  • Description
    Rabbit monoclonal [YE269] to Synaptophysin
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Donkey, Human
    Predicted to work with: Cow
  • Immunogen

    corresponding to Human Synaptophysin aa 250-350 (C terminal).
    (Peptide available as ab189853)

  • Positive control
    • WB: PC12 cell lysate, human fetal brain, mouse brain, rat brain IF: PC12 cells. IHC: Human pancreas, mouse cerebral cortex, rat cerebral cortex, medullablastoma, lung neuroendocrine tumor
  • General notes

     

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000 - 1/100000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).Can be blocked with Synaptophysin peptide (ab189853).
ICC/IF 1/50 - 1/250.

 

IHC-Fr Use at an assay dependent concentration.
IHC-P 1/400 - 1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

Target

  • FunctionPossibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • Tissue specificityCharacteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • Involvement in diseaseMental retardation, X-linked, SYP-related
  • Sequence similaritiesBelongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • DomainThe calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • Post-translational
    modifications
    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localizationCytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

Anti-Synaptophysin antibody [YE269] images

  • Immunofluorescent staining of PC-12 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab32127 at a dilution of 1/50. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/500 and the cells were counter stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, Alex Fluor® 594 goat anti-mouse was used at a dilution of 1/500.

  • Unpurified ab32127 showing positive staining in Medulloblastoma tissue.

  • All lanes : Anti-Synaptophysin antibody [YE269] (ab32127) at 1/100000 dilution (purified)

    Lane 1 : Mouse brain
    Lane 2 : Rat brain

    Secondary
    HRP goat anti-rabbit at 1/1000 dilution

    Predicted band size : 34 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab32127 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained wirh hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified ab32127 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained wirh hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemical staining of paraffin embedded human pancreas with purified ab32127 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained wirh hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Anti-Synaptophysin antibody [YE269] (ab32127) at 1/100000 dilution (purified) + Human fetal brain at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 34 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-Synaptophysin antibody [YE269] (ab32127) at 1/10000 dilution (unpurified) + PC12 cell lysate

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional bands at : 21 kDa,22 kDa. We are unsure as to the identity of these extra bands.
  • Unpurified ab32127 showing positive staining in Lung neuroendocrine tumor tissue.

References for Anti-Synaptophysin antibody [YE269] (ab32127)

This product has been referenced in:
  • Jiang T  et al. TREM2 Overexpression has No Improvement on Neuropathology and Cognitive Impairment in Aging APPswe/PS1dE9 Mice. Mol Neurobiol 54:855-865 (2017). WB ; Mouse . Read more (PubMed: 26780455) »
  • Bellissimo T  et al. Thymic Epithelial Tumors phenotype relies on miR-145-5p epigenetic regulation. Mol Cancer 16:88 (2017). WB ; Human . Read more (PubMed: 28486946) »

See all 23 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Whole brain tissue lysate)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (3-8% Tris-Acetate)
Loading amount 20 µg
Specification Whole brain tissue lysate
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Jun 05 2017

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Whole brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization Yes - 0.05% Tween-20
Specification Whole brain
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Paraformaldehyde
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Submitted Jun 05 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Horse Cell (Cerebellum)
Permeabilization No
Specification Cerebellum
Fixative None
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Stephen Cahalan

Verified customer

Submitted May 12 2017

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Horse Tissue sections (Adrenal gland)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Specification Adrenal gland
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 22°C
Fixative Formaldehyde
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Submitted Feb 06 2017

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (pancreas)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: CC1
Permeabilization No
Specification pancreas
Fixative formalin 4%
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Dr. Emmanuel Bouchaert

Verified customer

Submitted Jan 17 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE pH9
Permeabilization No
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% Normal buffered formalin
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Submitted Dec 05 2016

Application IHC - Wholemount
Sample Mouse Tissue (Diaphragm)
Specification Diaphragm
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Submitted Oct 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (neurons from iPS cells)
Gel Running Conditions Non-reduced Denaturing (12%)
Loading amount 20 µg
Specification neurons from iPS cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Jan 15 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Rat Tissue sections (Spinal cord)
Specification Spinal cord
Permeabilization Yes - 0.1% triton x-100
Fixative Paraformaldehyde
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Submitted Oct 06 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Donkey Tissue sections (brain slice)
Specification brain slice
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
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Submitted Sep 08 2014

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