The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 118 kDa. Additional lower bands may be observed which may represent degradation products. The staining can be blocked by the immunizing peptide.
Use a concentration of 1 - 2 µg/ml.
FunctionHas an actin-binding and actin-bundling activity.
Tissue specificitySkeletal muscle-specific.
Sequence similaritiesBelongs to the synaptopodin family. Contains 1 PDZ (DHR) domain.
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm in a differentiation-dependent and stress-induced fashion. Localizes to the Z-disk in mature striated muscle. The nuclear export is XPO1-dependent (By similarity). Localized in a fiber-like pattern. partly overlapping with filamentous actin.
References for Anti-Synaptopodin 2 antibody (ab50192)
This product has been referenced in:
Kai F et al. Myopodin isoforms alter the chemokinetic response of PC3 cells in response to different migration stimuli via differential effects on Rho-ROCK signaling pathways. Carcinogenesis33:2100-7 (2012).
Read more (PubMed: 22915763) »