The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 99 kDa.
Use at an assay dependent concentration.
Use a concentration of 5 µg/ml.
FunctionActin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity.
Tissue specificityExpressed in cerebral cortex.
Sequence similaritiesBelongs to the synaptopodin family.
Post-translational modificationsO-glycosylated. Isoform 2 is phosphorylated on Ser-826, Thr-836, Ser-840 and Ser-871.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > tight junction. Perikaryon. Cell projection > dendritic spine. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Asssociated with dendritic spines of a subset of synapses.