Overview

  • Product name
    Anti-Synaptopodin antibody
    See all Synaptopodin primary antibodies
  • Description
    Rabbit polyclonal to Synaptopodin
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide:

    GQRSPKLSQEALQTGHPSS

    conjugated to KLH, corresponding to internal sequence amino acids 184-202 of Rat Synaptopodin

  • Positive control
    • Cytosolic S1 fraction of rat brain.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Whole antiserum was fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Minimum dilution 1/3000. Predicted molecular weight: 100 kDa.
    Staining of synaptopodin in immunoblotting is specifically inhibited with the
    synaptopodin immunizing peptide (rat, amino acids 184-202).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity.
    • Tissue specificity
      Expressed in cerebral cortex.
    • Sequence similarities
      Belongs to the synaptopodin family.
    • Post-translational
      modifications
      O-glycosylated.
      Isoform 2 is phosphorylated on Ser-826, Thr-836, Ser-840 and Ser-871.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cell junction > tight junction. Perikaryon. Cell projection > dendritic spine. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Asssociated with dendritic spines of a subset of synapses.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA1029 antibody
      • Synaptopodin antibody
      • SYNPO antibody
      • SYNPO_HUMAN antibody

    References for Anti-Synaptopodin antibody (ab50859)

    This product has been referenced in:
    • Moriguchi T  et al. Gata3 Hypomorphic Mutant Mice Rescued with a Yeast Artificial Chromosome Transgene Suffer a Glomerular Mesangial Cell Defect. Mol Cell Biol 36:2272-81 (2016). IHC ; Mouse . Read more (PubMed: 27296697) »
    • Reissner KJ  et al. AKAP signaling in reinstated cocaine seeking revealed by iTRAQ proteomic analysis. J Neurosci 31:5648-58 (2011). WB ; Rat . Read more (PubMed: 21490206) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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