Purification notesWhole antiserum was fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Minimum dilution 1/3000. Predicted molecular weight: 100 kDa.
Staining of synaptopodin in immunoblotting is specifically inhibited with the
synaptopodin immunizing peptide (rat, amino acids 184-202).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionActin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity.
Tissue specificityExpressed in cerebral cortex.
Sequence similaritiesBelongs to the synaptopodin family.
Post-translational modificationsO-glycosylated. Isoform 2 is phosphorylated on Ser-826, Thr-836, Ser-840 and Ser-871.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > tight junction. Perikaryon. Cell projection > dendritic spine. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Asssociated with dendritic spines of a subset of synapses.