Overview

  • Product nameAnti-Synaptopodin antibody
    See all Synaptopodin primary antibodies
  • Description
    Rabbit polyclonal to Synaptopodin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide:

    GQRSPKLSQEALQTGHPSS

    conjugated to KLH, corresponding to internal sequence amino acids 184-202 of Rat Synaptopodin

  • Positive control
    • Cytosolic S1 fraction of rat brain.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesWhole antiserum was fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Minimum dilution 1/3000. Predicted molecular weight: 100 kDa.
    Staining of synaptopodin in immunoblotting is specifically inhibited with the
    synaptopodin immunizing peptide (rat, amino acids 184-202).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity.
    • Tissue specificityExpressed in cerebral cortex.
    • Sequence similaritiesBelongs to the synaptopodin family.
    • Post-translational
      modifications
      O-glycosylated.
      Isoform 2 is phosphorylated on Ser-826, Thr-836, Ser-840 and Ser-871.
    • Cellular localizationCytoplasm > cytoskeleton. Cell junction > tight junction. Perikaryon. Cell projection > dendritic spine. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Asssociated with dendritic spines of a subset of synapses.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA1029 antibody
      • Synaptopodin antibody
      • SYNPO antibody
      • SYNPO_HUMAN antibody

    References for Anti-Synaptopodin antibody (ab50859)

    This product has been referenced in:
    • Reissner KJ  et al. AKAP signaling in reinstated cocaine seeking revealed by iTRAQ proteomic analysis. J Neurosci 31:5648-58 (2011). WB ; Rat . Read more (PubMed: 21490206) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"