Overview

  • Product name
    Anti-Synaptotagmin 1 antibody
    See all Synaptotagmin 1 primary antibodies
  • Description
    Rabbit polyclonal to Synaptotagmin 1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Rabbit, Chicken, Guinea pig, Cow, Human, Pig, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse Synaptotagmin 1.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Forebrain; Mouse Hippocampus; Mouse Cerebellum; Mouse Olfactory bulb; Rat Brain; Rat Hippocampus.

Properties

Applications

Our Abpromise guarantee covers the use of ab106621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa).

Target

  • Function
    May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
  • Sequence similarities
    Belongs to the synaptotagmin family.
    Contains 2 C2 domains.
  • Domain
    The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
    The second C2 domain mediates interaction with SV2A and STN2.
  • Cellular localization
    Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Cytoplasm. Synaptic vesicles and chromaffin granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781D2042 antibody
    • FLJ42519 antibody
    • p65 antibody
    • SVP65 antibody
    • Synaptotagmin 2 antibody
    • Synaptotagmin I antibody
    • Synaptotagmin II antibody
    • Synaptotagmin-1 antibody
    • SYT antibody
    • Syt1 antibody
    • SYT1_HUMAN antibody
    • Syt2 antibody
    • SytI antibody
    see all

Images

  • All lanes : Anti-Synaptotagmin 1 antibody (ab106621) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Forebrain (Mouse) Tissue Lysate
    Lane 3 : Hippocampus (Mouse) Tissue Lysate
    Lane 4 : Cerebellum Mouse Tissue Lysate
    Lane 5 : Mouse Olfactory Bulb Lysate
    Lane 6 : Brain (Rat) Tissue Lysate
    Lane 7 : Rat Hippocampus Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References

This product has been referenced in:

See 1 Publication for this product

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