Anti-Synaptotagmin 1 antibody (ab53484)

Overview

  • Product nameAnti-Synaptotagmin 1 antibody
    See all Synaptotagmin 1 primary antibodies
  • Description
    Chicken polyclonal to Synaptotagmin 1
  • Specificityab53484 recognizes Synaptotagmin 1.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Apteronotus leptorhynchus
  • Immunogen

    Four synthetic peptides conjugated to KLH, corresponding to different regions of the Synaptotagmin 1 gene product, but are shared between the human (NP_005630, NCBI) and mouse (NP_033332, NCBI) sequences.

Properties

Applications

Our Abpromise guarantee covers the use of ab53484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notesICC/IF: 1/100.
    WB: 1/1000. Predicted molecular weight: 48 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
    • Sequence similaritiesBelongs to the synaptotagmin family.
      Contains 2 C2 domains.
    • DomainThe first C2 domain mediates Ca(2+)-dependent phospholipid binding.
      The second C2 domain mediates interaction with SV2A and STN2.
    • Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Cytoplasm. Synaptic vesicles and chromaffin granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp781D2042 antibody
      • FLJ42519 antibody
      • p65 antibody
      • SVP65 antibody
      • Synaptotagmin 2 antibody
      • Synaptotagmin I antibody
      • Synaptotagmin II antibody
      • Synaptotagmin-1 antibody
      • SYT antibody
      • Syt1 antibody
      • SYT1_HUMAN antibody
      • Syt2 antibody
      • SytI antibody
      see all

    Anti-Synaptotagmin 1 antibody images

    References for Anti-Synaptotagmin 1 antibody (ab53484)

    ab53484 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Apteronotus leptorhynchus Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization Yes - 0.3% Triton X-100
    Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 26 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount 50 µg
    Specification Brain
    Gel Running Conditions Reduced Denaturing (4-15%)
    Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 26 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"