Overview

  • Product nameAnti-Synaptotagmin 1 antibodySee all Synaptotagmin 1 primary antibodies ...
  • Description
    Mouse monoclonal to Synaptotagmin 1
  • Tested applicationsFlow Cyt, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length human Synaptotagmin 1 protein (AAH58917) with a 26kDa tag

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5µg for 106 cells.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 48 kDa.
ELISA Use at an assay dependent dilution. Detection limit is approximately 3 ng/ml as a capture antibody when used against the immunogen.

Target

  • FunctionMay have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
  • Sequence similaritiesBelongs to the synaptotagmin family.
    Contains 2 C2 domains.
  • DomainThe first C2 domain mediates Ca(2+)-dependent phospholipid binding.
    The second C2 domain mediates interaction with SV2A and STN2.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Cytoplasm. Synaptic vesicles and chromaffin granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781D2042 antibody
    • P65 antibody
    • SVP65 antibody
    • Synaptotagmin I antibody
    • Synaptotagmin-1 antibody
    • SYT antibody
    • SYT1 antibody
    • SYT1_HUMAN antibody
    • SytI antibody
    see all

Anti-Synaptotagmin 1 antibody images

  • Anti-Synaptotagmin 1 antibody (ab77314) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    developed using the ECL technique

    Predicted band size : 48 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Anti-Synaptotagmin 1 antibody (ab77314) at 5 µg/ml + Jurkat cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    developed using the ECL technique

    Predicted band size : 48 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • ab77314 was used as a capture antibody at 3ng/ml followed by incubation with the immunogen (recombinant protein) at the indicated concentration.
  • Overlay histogram showing SH-SY5Y cells stained with ab77314 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77314, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Synaptotagmin 1 antibody (ab77314)

This product has been referenced in:
  • Nishino J  et al. A network of heterochronic genes including Imp1 regulates temporal changes in stem cell properties. Elife 2:e00924 (2013). WB ; Mouse . Read more (PubMed: 24192035) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - other (Purified Brain Synaptosomes OF HIPPOCAMPUS)
Loading amount 40 µg
Specification Purified Brain Synaptosomes OF HIPPOCAMPUS
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Ms. Shalini Saggu

Verified customer

Submitted May 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"