Overview

  • Product nameAnti-Synaptotagmin 1 antibody
    See all Synaptotagmin 1 primary antibodies
  • Description
    Mouse monoclonal to Synaptotagmin 1
  • Tested applicationsFlow Cyt, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length human Synaptotagmin 1 protein (AAH58917) with a 26kDa tag

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5-1µg for 106 cells. Ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 48 kDa.
ELISA Use at an assay dependent concentration. Detection limit is approximately 3 ng/ml as a capture antibody when used against the immunogen.

Target

  • FunctionMay have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
  • Sequence similaritiesBelongs to the synaptotagmin family.
    Contains 2 C2 domains.
  • DomainThe first C2 domain mediates Ca(2+)-dependent phospholipid binding.
    The second C2 domain mediates interaction with SV2A and STN2.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Cytoplasm. Synaptic vesicles and chromaffin granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781D2042 antibody
    • FLJ42519 antibody
    • P65 antibody
    • SVP65 antibody
    • Synaptotagmin 2 antibody
    • Synaptotagmin I antibody
    • Synaptotagmin II antibody
    • Synaptotagmin-1 antibody
    • SYT antibody
    • SYT1 antibody
    • SYT1_HUMAN antibody
    • Syt2 antibody
    • SytI antibody
    see all

Anti-Synaptotagmin 1 antibody images

  • Anti-Synaptotagmin 1 antibody (ab77314) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    developed using the ECL technique

    Predicted band size : 48 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    The observed band size may not correspond to the predicted protein molecular weight as the immunogen (recombinant tagged protein) was used as the sample. Molecular weight of the tag alone is 26 kDa.
  • Anti-Synaptotagmin 1 antibody (ab77314) at 5 µg/ml + Jurkat cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    developed using the ECL technique

    Predicted band size : 48 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • ab77314 was used as a capture antibody at 3ng/ml followed by incubation with the immunogen (recombinant protein) at the indicated concentration.
  • Overlay histogram showing SH-SY5Y cells stained with ab77314 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77314, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Synaptotagmin 1 antibody (ab77314)

This product has been referenced in:
  • Nishino J  et al. A network of heterochronic genes including Imp1 regulates temporal changes in stem cell properties. Elife 2:e00924 (2013). WB ; Mouse . Read more (PubMed: 24192035) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (Purified Brain Synaptosomes OF HIPPOCAMPUS)
Loading amount 40 µg
Specification Purified Brain Synaptosomes OF HIPPOCAMPUS
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Ms. Shalini Saggu

Verified customer

Submitted May 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"