Overview

  • Product nameAnti-Synaptotagmin 4 antibody
    See all Synaptotagmin 4 primary antibodies
  • Description
    Mouse monoclonal to Synaptotagmin 4
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MAPITTSREE FDEIPTVVGI FSAFGLVFTV SLFAWICCQR KSSKSNKTPP YKFVHVLKGV DIYPENLNSK KKFGADDKNE VKNKPAVPKN SLHLDLEKR, corresponding to amino acids 1-100 of Human Synaptotagmin 4

Properties

Applications

Our Abpromise guarantee covers the use of ab57473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IHC-Fr
  • Application notesICC/IF: Use at an assay dependent dilution (PMID 19448629).
    IHC-Fr: Use at an assay dependent dilution (PMID 20010821).
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 48 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceSynaptotagmin 4 (SYT4) belongs to the synaptotagmin family of multi-domained, integral membrane proteins of synaptic vesicles. It may be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. It is primarily expressed in the brain.
    • Cellular localizationCytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.
    • Database links
    • Alternative names
      • HsT1192 antibody
      • KIAA1342 antibody
      • Synaptotagmin IV antibody
      • Synaptotagmin4 antibody
      • SynaptotagminIV antibody
      • SYT 4 antibody
      • Syt IV antibody
      • SYT4 antibody
      • SytIV antibody
      see all

    Anti-Synaptotagmin 4 antibody images



    • Predicted band size : 48 kDa
    • ICC/IF image of ab57473 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57473, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-Synaptotagmin 4 antibody (ab57473)

    This product has been referenced in:
    • Johnson SL  et al. Synaptotagmin IV determines the linear Ca2+ dependence of vesicle fusion at auditory ribbon synapses. Nat Neurosci 13:45-52 (2010). IHC-Fr ; Mouse, Gerbil . Read more (PubMed: 20010821) »
    • Dean C  et al. Synaptotagmin-IV modulates synaptic function and long-term potentiation by regulating BDNF release. Nat Neurosci 12:767-76 (2009). WB, ICC/IF . Read more (PubMed: 19448629) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab57473.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"