Anti-Synaptotagmin antibody [ASV30] (ab13259)

Overview

  • Product nameAnti-Synaptotagmin antibody [ASV30]See all Synaptotagmin primary antibodies ...
  • Description
    Mouse monoclonal [ASV30] to Synaptotagmin
  • Tested applicationsWB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Rat brain synaptic junctional protein complexes.

  • Positive control
    • Mouse or Rat brain tissue extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab13259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
IP Use a concentration of 5 µg/ml.

Target

  • RelevanceThe synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first C2 domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • DKFZp781D2042 antibody
    • P65 antibody
    • P65 antibody
    • SVP65 antibody
    • Synaptotagmin I antibody
    • Synaptotagmin II antibody
    • Synaptotagmin IV antibody
    • Synaptotagmin V antibody
    • SYT 1 antibody
    • SYT antibody
    • SYT1 antibody
    • SYT2 antibody
    • SYT3 antibody
    • SYT4 antibody
    • SYT5 antibody
    • SytI antibody
    see all

Anti-Synaptotagmin antibody [ASV30] images

  • All lanes : Anti-Synaptotagmin antibody [ASV30] (ab13259) at 1/1000 dilution

    Lane 1 : Molecular weight ladder
    Lane 2 : Lysates prepared from mouse brain
    Lane 3 : Lysates prepared from rat brain
  • ICC/IF image of ab13259 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13259, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Synaptotagmin was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Mouse monoclonal to Synaptotagmin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab13259.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
    Band: 64kDa; Synaptotagmin

References for Anti-Synaptotagmin antibody [ASV30] (ab13259)

This product has been referenced in:
  • Wong FK  et al. Synaptic vesicle tethering and the CaV2.2 distal C-terminal. Front Cell Neurosci 8:71 (2014). WB ; Chicken . Read more (PubMed: 24639630) »
  • Wong FK & Stanley EF Rab3a interacting molecule (RIM) and the tethering of pre-synaptic transmitter release site-associated CaV2.2 calcium channels. J Neurochem 112:463-73 (2010). WB, IP ; Chicken . Read more (PubMed: 19878533) »

See all 3 Publications for this product

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Application Western blot
Sample Rat Cell lysate - other (Post nuclear rat brain lysate)
Loading amount 25 µg
Specification Post nuclear rat brain lysate
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 16°C
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Dr. sriharsha kantamneni

Verified customer

Submitted Sep 26 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"