The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 0.1 µg/ml. Sufficient for detection of Synaptotagmin in 20µg of rat brain tissue extract by colorimetric immunoblot analysis.
Predicted molecular weight: 47 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
Sequence similaritiesBelongs to the synaptotagmin family. Contains 2 C2 domains.
DomainThe first C2 domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2.