Anti-Synaptotagmin (phospho T202) antibody (ab51182)

Overview

  • Product nameAnti-Synaptotagmin (phospho T202) antibody
    See all Synaptotagmin primary antibodies
  • Description
    Rabbit polyclonal to Synaptotagmin (phospho T202)
  • Specificityab51182 detects endogenous levels of Synaptotagmin only when phosphorylated at threonine 202.
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human Synaptotagmin around the phosphorylation site of threonine 202(R-K-TP-L-N).

  • Positive control
    • WB: Extracts from Hela cells. IHC-P: Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab51182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 48 kDa.

Target

Anti-Synaptotagmin (phospho T202) antibody images

  • Anti-Synaptotagmin (phospho T202) antibody (ab51182) at 1/500 dilution + extracts from Hela cells treated with Forskolin (40nM, 30min)

    Predicted band size : 48 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
  • ab51182, at dilution of 1/50, staining Synaptotagmin in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
    Left image: No peptide.
    Right image: Phosphopeptide present.

References for Anti-Synaptotagmin (phospho T202) antibody (ab51182)

ab51182 has not yet been referenced specifically in any publications.

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