The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: 1/2000. Predicted molecular weight: 57 kDa.
This antibody gave a positive result in Western blot against the immunising peptide (ab24250).
Not yet tested in other applications.
FunctionMay be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. May mediate Ca(2+)-regulation of exocytosis in acrosomal reaction in sperm.
Sequence similaritiesBelongs to the synaptotagmin family. Contains 2 C2 domains.
Western blot - Synaptotagmin VI antibody (ab24250)
All lanes : Anti-Synaptotagmin VI antibody (ab24250) at 1/2000 dilution
Lane 1 : SH-SY5Y cell lysate. Lane 2 : Mouse liver tissue. Lane 3 : Rat liver tissue
Predicted band size : 57 kDa
References for Anti-Synaptotagmin VI antibody (ab24250)
This product has been referenced in:
Suhaiman L et al. Sphingosine 1-phosphate and sphingosine kinase are involved in a novel signaling pathway leading to acrosomal exocytosis. J Biol Chem285:16302-14 (2010).
Read more (PubMed: 20236935) »