Overview

  • Product name
    Anti-Synaptotagmin VII antibody
    See all Synaptotagmin VII primary antibodies
  • Description
    Rabbit polyclonal to Synaptotagmin VII
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Rabbit, Human, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Synaptotagmin VII.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cortex; Rat Cortex.

Properties

Applications

Our Abpromise guarantee covers the use of ab106618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). Abcam recommends using milk as the blocking agent - 3%.

Target

  • Function
    May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis.
  • Tissue specificity
    Expressed in a variety of adult and fetal tissues.
  • Sequence similarities
    Belongs to the synaptotagmin family.
    Contains 2 C2 domains.
  • Domain
    The second C2 domain/C2B is responsible for SYNCRIP binding.
  • Cellular localization
    Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • IPCA 7 antibody
    • IPCA-7 antibody
    • IPCA7 antibody
    • PCANAP7 antibody
    • prostate cancer associated protein 7 antibody
    • Prostate cancer-associated protein 7 antibody
    • Synaptotagmin VII antibody
    • Synaptotagmin-7 antibody
    • Synaptotagmin7 antibody
    • SynaptotagminVII antibody
    • Synatotagmin 7 antibody
    • SYT 7 antibody
    • SYT VII antibody
    • SYT7 antibody
    • SYT7_HUMAN antibody
    • SytVII antibody
    see all

Images

  • All lanes : Anti-Synaptotagmin VII antibody (ab106618) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse Cortex Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Rat Cortex Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 45,63,68 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 43 kDa, 76 kDa, 78 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes


    The bands observed are representative of Synaptotagmin VII splice variants alpha, beta, and delta. While these differ to its predicted molecular weight of 45 kDa, this migration has been observed in the literature (PMID:17709608) This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab106618 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab106618 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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