This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cortex; Rat Cortex.
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Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). Abcam recommends using milk as the blocking agent - 3%.
May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis.
Expressed in a variety of adult and fetal tissues.
Belongs to the synaptotagmin family. Contains 2 C2 domains.
The second C2 domain/C2B is responsible for SYNCRIP binding.
The bands observed are representative of Synaptotagmin VII splice variants alpha, beta, and delta. While these differ to its predicted molecular weight of 45 kDa, this migration has been observed in the literature (PMID:17709608)
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab106618 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.