Overview

  • Product name
    Anti-Synaptotagmin VII antibody
    See all Synaptotagmin VII primary antibodies
  • Description
    Rabbit polyclonal to Synaptotagmin VII
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Rabbit, Human, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Synaptotagmin VII.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cortex; Rat Cortex.

Properties

Applications

Our Abpromise guarantee covers the use of ab106618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). Abcam recommends using milk as the blocking agent - 3%.

Target

  • Function
    May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis.
  • Tissue specificity
    Expressed in a variety of adult and fetal tissues.
  • Sequence similarities
    Belongs to the synaptotagmin family.
    Contains 2 C2 domains.
  • Domain
    The second C2 domain/C2B is responsible for SYNCRIP binding.
  • Cellular localization
    Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • IPCA 7 antibody
    • IPCA-7 antibody
    • IPCA7 antibody
    • PCANAP7 antibody
    • prostate cancer associated protein 7 antibody
    • Prostate cancer-associated protein 7 antibody
    • Synaptotagmin VII antibody
    • Synaptotagmin-7 antibody
    • Synaptotagmin7 antibody
    • SynaptotagminVII antibody
    • Synatotagmin 7 antibody
    • SYT 7 antibody
    • SYT VII antibody
    • SYT7 antibody
    • SYT7_HUMAN antibody
    • SytVII antibody
    see all

Images

  • All lanes : Anti-Synaptotagmin VII antibody (ab106618) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse Cortex Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Rat Cortex Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45,63,68 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 43 kDa,76 kDa,78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References

ab106618 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab106618.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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