Anti-Synaptotagmin VII antibody (ab121383)
Key features and details
- Rabbit polyclonal to Synaptotagmin VII
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Synaptotagmin VII antibody
See all Synaptotagmin VII primary antibodies -
Description
Rabbit polyclonal to Synaptotagmin VII -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen, corresponding to amino acids 51-181 of Human Synaptotagmin VII.
-
Positive control
- Synaptotagmin VII-transfected HEK293T lysate; Human kidney tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
Notes |
---|
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
-
Function
May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. -
Tissue specificity
Expressed in a variety of adult and fetal tissues. -
Sequence similarities
Belongs to the synaptotagmin family.
Contains 2 C2 domains. -
Domain
The second C2 domain/C2B is responsible for SYNCRIP binding. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. - Information by UniProt
-
Database links
- Entrez Gene: 9066 Human
- Omim: 604146 Human
- SwissProt: O43581 Human
- Unigene: 502730 Human
- Unigene: 684589 Human
-
Alternative names
- IPCA 7 antibody
- IPCA-7 antibody
- IPCA7 antibody
see all
Images
-
All lanes : Anti-Synaptotagmin VII antibody (ab121383) at 1/250 dilution
Lane 1 : vector only transfected HEK293T lysate
Lane 2 : Synaptotagmin VII-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
10-20% SDS PAGE -
ab121383 at 1/25 dilution staining Synaptotagmin VII in Paraffin-embedded Human kidney tissue by Immunohistochemistry, showing strong cytoplasmic positivity, with a granular pattern, in cells in tubules.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab121383 has been referenced in 2 publications.
- Han Q et al. High SYT7 expression is associated with poor prognosis in human non-small cell lung carcinoma. Pathol Res Pract 216:153101 (2020). PubMed: 32825966
- Liu X et al. Synaptotagmin 7 in twist-related protein 1-mediated epithelial - Mesenchymal transition of non-small cell lung cancer. EBioMedicine 46:42-53 (2019). PubMed: 31395502