Anti-Syndecan-1 antibody (ab60199)
Key features and details
- Rabbit polyclonal to Syndecan-1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Syndecan-1 antibody
See all Syndecan-1 primary antibodies -
Description
Rabbit polyclonal to Syndecan-1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide (Human) surrounding amino acid 298
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Positive control
- Mouse tissue lysate
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 3643R Syndecan-1 Antibody. 3643R-100 is the same size as the 100 µg size of ab60199.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab60199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 34 kDa).
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Notes |
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WB
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 34 kDa). |
Target
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Function
Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. -
Sequence similarities
Belongs to the syndecan proteoglycan family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 20969 Mouse
- SwissProt: P18828 Mouse
- Unigene: 2580 Mouse
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Alternative names
- CD_antigen antibody
- CD138 antibody
- CD138 antigen antibody
see all
Images
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Anti-Syndecan-1 antibody (ab60199) at 4 µg/ml + Mouse tissue lysate
Predicted band size: 34 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
The observed band size is larger than predicted due to haparan sulfate and chondroitin sulfate modifications. -
Anti-Syndecan-1 antibody (ab60199) at 1/2000 dilution + lysate prepared from ScN2a cells.
Secondary
peroxidase-conjugated secondary
Developed using the ECL technique.
Predicted band size: 34 kDa
Samples were prepared in dissociation buffer and boiled for 5 minutes. Proteins were resolved by SDS-PAGE using either 7–17% polyacrylamide gradient gels or 14.5% polyacrylamide gels. For western blot analysis, resolved proteins were transferred to Immobilon P polyvinylidene difluoride membrane. The membrane was blocked by incubation for 1 hour with phosphate-buffered saline containing 0.1% (v/v) Tween-20 and 5% (w/v) dried milk powder. Antibody incubations were performed in phosphate-buffered saline-Tween containing 2% (v/v) bovine serum albumin. Horseradish peroxidase-conjugated streptavidin was used at 1/2000.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab60199 has been referenced in 9 publications.
- Cao G et al. circCEP128 Knockdown Suppresses Bladder Cancer Progression via Regulating microRNA-515-5p/SDC1 Axis. Cancer Manag Res 13:2885-2896 (2021). PubMed: 33833571
- Yu CG et al. Inhibition of Bruton Tyrosine Kinase Reduces Neuroimmune Cascade and Promotes Recovery after Spinal Cord Injury. Int J Mol Sci 23:N/A (2021). PubMed: 35008785
- Jing Z et al. Phosphorylation of Dab2 is involved in inhibited VEGF-VEGFR-2 signaling induced by downregulation of syndecan-1 in glomerular endothelial cell. Cell Biol Int 44:894-904 (2020). PubMed: 31868265
- Li H et al. Interferon-induced mechanosensing defects impede apoptotic cell clearance in lupus. J Clin Invest 125:2877-90 (2015). PubMed: 26098211
- Boeddeker SJ et al. Syndecan-1 knockdown in endometrial epithelial cells alters their apoptotic protein profile and enhances the inducibility of apoptosis. Mol Hum Reprod N/A:N/A (2014). Human . PubMed: 24478463
- Chaterji S et al. Syndecan-1 regulates vascular smooth muscle cell phenotype. PLoS One 9:e89824 (2014). Mouse . PubMed: 24587062
- Park PJ et al. An investigative peptide-acyclovir combination to control herpes simplex virus type 1 ocular infection. Invest Ophthalmol Vis Sci 54:6373-81 (2013). PubMed: 23989188
- Cassano M et al. Alpha sarcoglycan is required for FGF-dependent myogenic progenitor cell proliferation in vitro and in vivo. Development 138:4523-33 (2011). WB ; Mouse . PubMed: 21903674
- Taylor DR et al. Glypican-1 mediates both prion protein lipid raft association and disease isoform formation. PLoS Pathog 5:e1000666 (2009). WB ; Mouse . PubMed: 19936054