Anti-Syndecan-1 antibody [B-A38] (ab82200)

Overview

  • Product nameAnti-Syndecan-1 antibody [B-A38]
    See all Syndecan-1 primary antibodies
  • Description
    Mouse monoclonal [B-A38] to Syndecan-1
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human): U266 cell line

  • Positive control
    • Tonsil tissue

Applications

Our Abpromise guarantee covers the use of ab82200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/20 - 1/50.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionCell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.
  • Sequence similaritiesBelongs to the syndecan proteoglycan family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD_antigen antibody
    • CD138 antibody
    • CD138 antigen antibody
    • heparan sulfate proteoglycan fibroblast growth factor receptor antibody
    • SDC antibody
    • Sdc1 antibody
    • SDC1_HUMAN antibody
    • SYND1 antibody
    • Syndecan 1 antibody
    • syndecan proteoglycan 1 antibody
    • Syndecan-1 antibody
    see all

Anti-Syndecan-1 antibody [B-A38] images

  • ab82200 staining Syndecan in human tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and then a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked using 5% serum for 1 hour at 22°C and then incubated with ab82200 at a 1/30 dilution for 20 hours at 4°C. The secondary antibody was a biotin conjugated rabbit anti-mouse monoclonal used at a 1/300 dilution.

    See Abreview

References for Anti-Syndecan-1 antibody [B-A38] (ab82200)

ab82200 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Aug 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"