Anti-Syndecan-1 antibody [EPR6454] (ab128936)

Overview

  • Product nameAnti-Syndecan-1 antibody [EPR6454]
    See all Syndecan-1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6454] to Syndecan-1
  • SpecificityBased on negative staining of mouse and rat spleen and stomach tissues in IHC-P we believe this antibody is unsuitable for IHC-P with mouse and rat. We believe the antibody is suitable for Western blot with mouse and rat based on data from brain, heart, kidney, and spleen lysates. Please contact our Scientific Support team for more information.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Syndecan-1 aa 1-300 (extracellular).
    Database link: P18827

  • Positive control
    • Human colon and cervical carcinoma tissues; Fetal kidney, HeLa, PC12, Raji, Ramos and BxPC-3 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Applications

Our Abpromise guarantee covers the use of ab128936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 2 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
IHC-P 1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified, use 1/500 - 1/1000.

  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    Anti-Syndecan-1 antibody [EPR6454] images

    • All lanes : Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/10000 dilution (purified)

      Lane 1 : Ramos whole cell lysate
      Lane 2 : Raji whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 32 kDa
      Observed band size : 35,100 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/5000 dilution (purified)

      Lane 1 : HeLa whole cell lysate
      Lane 2 : PC-12 whole cell lysate
      Lane 3 : Mouse heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 32 kDa
      Observed band size : 35,100 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin embedded human colon with purified ab128936 at a working dilution of 1/8000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Lanes 1 - 5 : Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/1000 dilution (Unpurified)
      Lane 6 : Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : BxPC-3 cell lysate
      Lane 5 : Fetal kidney lysate
      Lane 6 : PC12 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 32 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 65-100 kDa. We are unsure as to the identity of these extra bands.
    • Unpurified ab128936, at a 1/500 dilution, staining Syndecan in paraffin-embedded Human cervical carcinoma tissue by immunohistochemistry.

    • Unpurified ab128936, at a 1/500 dilution, staining Syndecan in paraffin-embedded Human colon tissue by immunohistochemistry.

    • Unpurified ab128936 showing positive staining in Normal skin tissue.

    • Unpurified ab128936 showing positive staining in normal tonsil tissue.

    • Unpurified ab128936 showing positive staining in Plasmacytoma tissue.

    • Unpurified ab128936 showing negative staining in Skeletal muscle tissue.

    References for Anti-Syndecan-1 antibody [EPR6454] (ab128936)

    ab128936 has not yet been referenced specifically in any publications.

    Product Wall

    Application Flow Cytometry
    Sample Human Cell (Human myeloma cell line)
    Permeabilization No
    Gating Strategy live cells
    Specification Human myeloma cell line
    Username

    Abcam user community

    Verified customer

    Submitted Jul 11 2016

    Application Immunohistochemistry (Resin sections)
    Sample Human Tissue sections (Human tonsil)
    Specification Human tonsil
    Username

    Abcam user community

    Verified customer

    Submitted Jan 07 2014

    I am very sorry to hear of the issues which you have been experiencing with this product. We do recommend use of heat mediated antigen retrieval but do understand that the traditional conditions, e.g. in pressure cooker of microwave, can be an issue wi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"