RabMAb

Anti-Syndecan-1 [EPR6454] antibody (ab128936)

Overview

  • Product nameAnti-Syndecan-1 [EPR6454] antibodySee all Syndecan-1 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR6454] to Syndecan-1
  • Tested applicationsIHC-R, WB, IHC-P, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Syndecan-1 aa 1-300 (extracellular).
    Database link: P18827

  • Positive control
    • Human colon and cervical carcinoma tissues; Fetal kidney, HeLa, PC12, Raji, Ramos and BxPC-3 cell lysates.
  • General notesProduced under U.S. Patent No. 5,675,063.

Applications

Our Abpromise guarantee covers the use of ab128936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-R Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/1000.
  • Application notesIs unsuitable for ICC or IP.
  • Target

    Anti-Syndecan-1 [EPR6454] antibody images

    • Overlay histogram showing Raji cells stained with ab128936 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab128936, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed Raji cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
    • All lanes : Anti-Syndecan-1 [EPR6454] antibody (ab128936) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : BxPC-3 cell lysate
      Lane 5 : Fetal kidney lysate
      Lane 6 : PC12 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 32 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 65-100 kDa. We are unsure as to the identity of these extra bands.
    • ab128936, at a 1/500 dilution, staining Syndecan in paraffin-embedded Human cervical carcinoma tissue by immunohistochemistry.
    • ab128936, at a 1/500 dilution, staining Syndecan in paraffin-embedded Human colon tissue by immunohistochemistry.
    • ab128936 showing positive staining in Normal skin tissue.

    • ab128936 showing positive staining in Normal tonsil tissue.

    • ab128936 showing positive staining in Plasmacytoma tissue.

    • ab128936 showing negative staining in Skeletal muscle tissue.

    References for Anti-Syndecan-1 [EPR6454] antibody (ab128936)

    ab128936 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Resin sections)
    Sample Human Tissue sections (Human tonsil)
    Specification Human tonsil
    Username

    Abcam user community

    Verified customer

    Submitted Jan 07 2014

    I am very sorry to hear of the issues which you have been experiencing with this product. We do recommend use of heat mediated antigen retrieval but do understand that the traditional conditions, e.g. in pressure cooker of microwave, can be an issue wi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"