All lanes :
Anti-Syndecan 2 antibody (ab79978) at 1 µg/mlLane 1 :
Raji (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 2 :
HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 :
Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 4 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 5 :
HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 6 :
LOVO (Human colon adenocarcinoma cell line) Whole Cell LysateLane 7 :
Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Observed band size:
18 kDa (why is the actual band size different from the predicted?
)Additional bands at:
31 kDa. We are unsure as to the identity of these extra bands.Exposure time:
The Syndecan 2 protein has a predicted molecular weight of 22 kDa. The first 18 amino acids of the syndecan 2 sequence act as a signal sequence, and when cleaved the protein has a molecular weight of 19 kDa (SwissProt), therefore explaining the bands presence at a lower than expected molecular weight.