Anti-Syndecan 3 antibody - N-terminal (ab191308)
Key features and details
- Rabbit polyclonal to Syndecan 3 - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Syndecan 3 antibody - N-terminal
See all Syndecan 3 primary antibodies -
Description
Rabbit polyclonal to Syndecan 3 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Chicken -
Immunogen
Synthetic peptide corresponding to Mouse Syndecan 3 aa 1-100 (N terminal).
Database link: Q64519 -
Positive control
- U87, 293T, PC12, NRK and HT1080 cell lysates; Rat brain tissue; Human placenta tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 46 kDa.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 46 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Cell surface proteoglycan that may bear heparan sulfate (By similarity). May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. -
Tissue specificity
Expressed in the nervous system, the adrenal gland, and the spleen. -
Sequence similarities
Belongs to the syndecan proteoglycan family. -
Post-translational
modificationsO-glycosylated within the Thr/Ser-rich region which could interact with lectin domains on other molecules. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 9672 Human
- Entrez Gene: 20970 Mouse
- Entrez Gene: 116673 Rat
- Omim: 186357 Human
- SwissProt: O75056 Human
- SwissProt: Q64519 Mouse
- SwissProt: P33671 Rat
- Unigene: 158287 Human
see all -
Alternative names
- N-syndecan antibody
- Sdc3 antibody
- SDC3_HUMAN antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Syndecan 3 with ab191308 at 1 μg/ml.
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Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling Syndecan 3 with ab191308 at 1 μg/ml.
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All lanes : Anti-Syndecan 3 antibody - N-terminal (ab191308) at 0.5 µg/ml
Lane 1 : U87 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NRK cell lysate
Lane 5 : HT1080 cell lysate
Predicted band size: 46 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab191308 has been referenced in 2 publications.
- Huang S et al. Myeloid differentiation 2 deficiency attenuates AngII-induced arterial vascular oxidative stress, inflammation, and remodeling. Aging (Albany NY) 13:4409-4427 (2021). PubMed: 33495414
- Santos NJ et al. Syndecan Family Gene and Protein Expression and Their Prognostic Values for Prostate Cancer. Int J Mol Sci 22:N/A (2021). PubMed: 34445387