Anti-Syndecan 3 antibody - N-terminal (ab191308)

Overview

  • Product name
    Anti-Syndecan 3 antibody - N-terminal
    See all Syndecan 3 primary antibodies
  • Description
    Rabbit polyclonal to Syndecan 3 - N-terminal
  • Host species
    Rabbit
  • Specificity
    No cross reactivity with other proteins.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken
  • Immunogen

    Synthetic peptide corresponding to Mouse Syndecan 3 aa 45-60 (N terminal).
    Sequence:

    AQRWRNENFERPVDLE


    Database link: Q64519

  • Positive control
    • U87, 293T, PC12, NRK and HT1080 cell lysates; Rat brain tissue; Human placenta tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 46 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Cell surface proteoglycan that may bear heparan sulfate (By similarity). May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.
  • Tissue specificity
    Expressed in the nervous system, the adrenal gland, and the spleen.
  • Sequence similarities
    Belongs to the syndecan proteoglycan family.
  • Post-translational
    modifications
    O-glycosylated within the Thr/Ser-rich region which could interact with lectin domains on other molecules.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • N-syndecan antibody
    • Sdc3 antibody
    • SDC3_HUMAN antibody
    • SDCN antibody
    • SYND3 antibody
    • Syndecan neural type antibody
    • syndecan proteoglycan 3 antibody
    • Syndecan-3 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Syndecan 3 with ab191308 at 1 μg/ml.

  • Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling Syndecan 3 with ab191308 at 1 μg/ml.

  • All lanes : Anti-Syndecan 3 antibody - N-terminal (ab191308) at 0.5 µg/ml

    Lane 1 : U87 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NRK cell lysate
    Lane 5 : HT1080 cell lysate

    Predicted band size: 46 kDa

References

ab191308 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191308.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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