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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Purity Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).
Function Cell surface proteoglycan that bears heparan sulfate.
Tissue specificity Expressed in epithelial and fibroblastic cells.
Sequence similarities Belongs to the syndecan proteoglycan family.
Post-translational modifications Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
Cellular localization Membrane. Secreted. Shedding of the ectodomain produces a soluble form.
Information by UniProt
Anti-Syndecan 4 antibody images
Western blot - Anti-Syndecan 4 antibody (ab104568)
All lanes : Anti-Syndecan 4 antibody (ab104568) at 1 µg/ml Lane 1 : STO (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 21 kDa Observed band size : 18 kDa ( why is the actual band size different from the predicted?) Exposure time : 20 minutes
References for Anti-Syndecan 4 antibody (ab104568)
has not yet been referenced specifically in any publications.
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