Overview

  • Product nameAnti-Syndecan 4 antibody
    See all Syndecan 4 primary antibodies
  • Description
    Rabbit polyclonal to Syndecan 4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Dog, Human, Pig, Baboon, Macaque Monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Syndecan 4.

  • Positive control
    • This antibody gave a positive signal in both STO and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab104568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).

Target

  • FunctionCell surface proteoglycan that bears heparan sulfate.
  • Tissue specificityExpressed in epithelial and fibroblastic cells.
  • Sequence similaritiesBelongs to the syndecan proteoglycan family.
  • Post-translational
    modifications
    Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
  • Cellular localizationMembrane. Secreted. Shedding of the ectodomain produces a soluble form.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amphiglycan antibody
    • MGC22217 antibody
    • OTTHUMP00000031788 antibody
    • ryudocan amphiglycan antibody
    • Ryudocan antibody
    • Ryudocan core protein antibody
    • SDC 4 antibody
    • Sdc4 antibody
    • SDC4_HUMAN antibody
    • SYND 4 antibody
    • SYND4 antibody
    • syndecan 4 antibody
    • syndecan 4 (amphiglycan, ryudocan) antibody
    • syndecan proteoglycan 4 antibody
    • Syndecan-4 antibody
    • Syndecan4 antibody
    see all

Anti-Syndecan 4 antibody images

  • All lanes : Anti-Syndecan 4 antibody (ab104568) at 1 µg/ml

    Lane 1 : STO (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

References for Anti-Syndecan 4 antibody (ab104568)

ab104568 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Pig Tissue lysate - whole (cardiac)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 40 µg
Specification cardiac
Blocking step Licor as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Feb 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"