Overview

  • Product name
    Anti-Syndecan 4 antibody
    See all Syndecan 4 primary antibodies
  • Description
    Rabbit polyclonal to Syndecan 4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Dog, Human, Pig, Baboon, Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Syndecan 4.

    Read Abcam's proprietary immunogen policy

  • Positive control
    • This antibody gave a positive signal in both STO and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab104568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).

Target

  • Function
    Cell surface proteoglycan that bears heparan sulfate.
  • Tissue specificity
    Expressed in epithelial and fibroblastic cells.
  • Sequence similarities
    Belongs to the syndecan proteoglycan family.
  • Post-translational
    modifications
    Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
  • Cellular localization
    Membrane. Secreted. Shedding of the ectodomain produces a soluble form.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Amphiglycan antibody
    • MGC22217 antibody
    • OTTHUMP00000031788 antibody
    • ryudocan amphiglycan antibody
    • Ryudocan antibody
    • Ryudocan core protein antibody
    • SDC 4 antibody
    • Sdc4 antibody
    • SDC4_HUMAN antibody
    • SYND 4 antibody
    • SYND4 antibody
    • syndecan 4 antibody
    • syndecan 4 (amphiglycan, ryudocan) antibody
    • syndecan proteoglycan 4 antibody
    • Syndecan-4 antibody
    • Syndecan4 antibody
    see all

Images

  • Western blot - Anti-Syndecan 4 antibody (ab104568)
    Western blot - Anti-Syndecan 4 antibody (ab104568)
    All lanes : Anti-Syndecan 4 antibody (ab104568) at 1 µg/ml

    Lane 1 : STO (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa
    Observed band size: 18 kDa (why is the actual band size different from the predicted?)


    Exposure time: 20 minutes


    The band observed at 18 kDa could potentially be a cleaved form of Syndecan 4 due to the presence of a 21 amino acid signal peptide.

References

ab104568 has not yet been referenced specifically in any publications.

Publishing research using ab104568? Please let us know so that we can cite the reference in this datasheet.

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Western blot abreview for Anti-Syndecan 4 antibody

 Inconclusive
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - whole (cardiac)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
40 µg
Specification
cardiac
Blocking step
Licor as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Feb 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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