- Datasheet
- Specific References
- Protocols
Overview
- Product nameAnti-Syndecan 4 antibody
See all Syndecan 4 primary antibodies - DescriptionRabbit polyclonal to Syndecan 4
- Tested applicationsSuitable for: WBmore details
- Species reactivityReacts with: Mouse
Predicted to work with: Rat, Cow, Dog, Human, Pig, Baboon, Macaque monkey, Orangutan
- Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Syndecan 4.
- Positive control
- This antibody gave a positive signal in both STO and NIH3T3 whole cell lysates.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... - PurityImmunogen affinity purified
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab104568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa). |
Target
- FunctionCell surface proteoglycan that bears heparan sulfate.
- Tissue specificityExpressed in epithelial and fibroblastic cells.
- Sequence similaritiesBelongs to the syndecan proteoglycan family.
- Post-translational
modificationsShedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3. - Cellular localizationMembrane. Secreted. Shedding of the ectodomain produces a soluble form.
- Information by UniProt
-
Database links
- Entrez Gene: 6385 Human
- Entrez Gene: 20971 Mouse
- Entrez Gene: 100174444 Orangutan
- Entrez Gene: 24771 Rat
- SwissProt: P31431 Human
- SwissProt: O35988 Mouse
- SwissProt: Q5RAT9 Orangutan
- SwissProt: P34901 Rat
see all -
Alternative names
- Amphiglycan antibody
- MGC22217 antibody
- OTTHUMP00000031788 antibody
see all
Anti-Syndecan 4 antibody images
-
Western blot - Anti-Syndecan 4 antibody (ab104568)All lanes : Anti-Syndecan 4 antibody (ab104568) at 1 µg/ml
Lane 1 : STO (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 21 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
References for Anti-Syndecan 4 antibody (ab104568)
ab104568 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"