Overview

  • Product nameAnti-Syndecan 4 antibody
    See all Syndecan 4 primary antibodies
  • Description
    Rabbit polyclonal to Syndecan 4
  • Specificityab74139 detects endogenous levels of total Syndecan 4 protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide from human Syndecan 4 around the phosphorylation site of serine 179 (E-G-SP-Y-D).

  • Positive control
    • Human brain tissue. Extracts from HepG2 cells. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA 1/10000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionCell surface proteoglycan that bears heparan sulfate.
  • Tissue specificityExpressed in epithelial and fibroblastic cells.
  • Sequence similaritiesBelongs to the syndecan proteoglycan family.
  • Post-translational
    modifications
    Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
  • Cellular localizationMembrane. Secreted. Shedding of the ectodomain produces a soluble form.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amphiglycan antibody
    • MGC22217 antibody
    • OTTHUMP00000031788 antibody
    • ryudocan amphiglycan antibody
    • Ryudocan antibody
    • Ryudocan core protein antibody
    • SDC 4 antibody
    • Sdc4 antibody
    • SDC4_HUMAN antibody
    • SYND 4 antibody
    • SYND4 antibody
    • syndecan 4 antibody
    • syndecan 4 (amphiglycan, ryudocan) antibody
    • syndecan proteoglycan 4 antibody
    • Syndecan-4 antibody
    • Syndecan4 antibody
    see all

Anti-Syndecan 4 antibody images

  • Immunohistochemistry analysis of human brain tissue using ab74139, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • All lanes : Anti-Syndecan 4 antibody (ab74139) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 22 kDa
    Observed band size : 22 kDa
  • Immunofluorescence analysis of HeLa cells using ab74139, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

References for Anti-Syndecan 4 antibody (ab74139)

ab74139 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"