Overview

  • Product nameAnti-SynGAP antibody
    See all SynGAP primary antibodies
  • Description
    Rabbit polyclonal to SynGAP
  • Tested applicationsSuitable for: IP, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Fusion protein corresponding to Rat SynGAP aa 947-1167.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesSynaptic Ras GTPase Activating Protein (SynGAP) is a PDZ-domain interacting protein expressed almost exclusively in the brain. SynGAP has been shown to be a major component of postsynaptic density and associates with post synaptic density 95 kDa (PSD-95), a synaptic scaffold protein, and synaptic N-methyl-D-aspartate (NMDA) receptors. SynGAP Ras-GTPase activity has also been shown to be inhibited by phosphorylation by CaM kinase II, another abundant protein located in the postsynaptic density. It has been proposed that SynGAP may function as a negative regulator of MAP Kinase activation in the absence of active NMDA receptors.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 148 kDa.

Target

  • FunctionMajor constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits.
  • Involvement in diseaseDefects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5) [MIM:612621]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD5 patients show global developmental delay with delayed motor development, hypotonia, moderate-to-severe mental retardation, and severe language impairment. Autism can be present in some patients.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 Ras-GAP domain.
  • Post-translational
    modifications
    Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp761G1421 antibody
    • KIAA1938 antibody
    • MRD5 antibody
    • Neuronal RasGAP antibody
    • OTTHUMP00000064825 antibody
    • p135 SynGAP antibody
    • Ras GTPase activating protein SynGAP antibody
    • Ras GTPase-activating protein SynGAP antibody
    • RASA 1 antibody
    • RASA 5 antibody
    • RASA1 antibody
    • RASA5 antibody
    • SYGP1_HUMAN antibody
    • Synaptic Ras GAP 1 antibody
    • Synaptic Ras GTPase activating protein 1 antibody
    • Synaptic Ras GTPase activating protein 1 homolog antibody
    • Synaptic Ras GTPase activating protein 135kDa antibody
    • Synaptic Ras GTPase activating protein antibody
    • Synaptic Ras GTPase-activating protein 1 antibody
    • Synaptic Ras-GAP 1 antibody
    • SYNGAP 1 antibody
    • SYNGAP1 antibody
    see all

Anti-SynGAP antibody images

  • Immunolocalization of SynGAP in rat hippocampal neurons using ab3344.

  • Immunolocalization of SynGAP in rat hippocampal neurons using ab3344.

References for Anti-SynGAP antibody (ab3344)

This product has been referenced in:
  • Yang Y  et al. SynGAP moves out of the core of the postsynaptic density upon depolarization. Neuroscience : (2011). WB, Electron Microscopy ; Rat . Read more (PubMed: 21736925) »

See 1 Publication for this product

Product Wall

Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (Various ovarian cancer cell lines)
Specification Various ovarian cancer cell lines
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"