Specificityab18011 identifies recombinant rat syntaxin 13 as an ~38kD protein (corresponding to the predicted band). Additional bands of 27 and 37kD may also be recognized and are believed to correspond to degradation products of syntaxin 13.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa.
Application notesIs unsuitable for ICC/IF.
RelevanceSyntaxin 13 is an integral membrane protein that belongs to the t-SNARE family, a group of proteins involved in protein transport. Confocal immunofluoresence and electron microscopy studies have shown that syntaxin 13 is primarily localized to tubular early and recycling endosomes, where it colocalizes with transferrin receptor, and it is also localized in endosomal vacuoles. Syntaxin 13 has been found to be expressed in all tissues, with higher levels of the protein found in brain, lung, spleen, thymus and testes. Immunoprecipitation studies show that syntaxin 13 complexes with beta-SNAP, VAMP2/3, and SNAP25. The binding of this complex to alpha-SNAP and NSF is terminated in the presence of ATP. These results suggest that syntaxin 13 is a SNARE protein which mediates the recycling protein flow through tubulo vesicular recycling endosomes.
Cellular localizationTubular early and recycling endosomes and endosomal vacuoles.
Dm Syx13 antibody
References for Anti-Syntaxin 13 antibody [4E212] (ab18011)
This product has been referenced in:
Chung WY et al. WNK4 inhibits plasma membrane targeting of NCC through regulation of syntaxin13 SNARE formation. Cell Signal25:2469-77 (2013).
Read more (PubMed: 23993962) »