(Peptide available as ab41452.)
On the basis of sequence homology, ab41453 is predicted to react with syntaxin 1B from a number of species, including rat, mouse, human, sheep, cow, zebrafish and chicken.
ab41453 gave a positive signal in the following tissue lysates:
Brain (Human); Brain (Mouse); Brain (Rat)
ab41453 also gave a positive signal in PC12 (Rat adrenal pheochromocytoma cell line) Cytoplasmic Lysate (data not shown)
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.
Involvement in disease
Note=STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.
ICC/IF image of ab41453 stained rat PC12 cells. The cells were methanol fixed (5 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab41453, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (PFA perfusion fixed frozen sections) - Syntaxin 1a antibody (ab41453)This image is courtesy of an Abreview submitted by Dr Sophie Pezet
ab41453 staining Syntaxin 1a in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/3000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Syntaxin 1a antibody (ab41453)Image from Zhang HH et al., PLoS One. 2012;7(10):e46666. doi: 10.1371/journal.pone.0046666. Epub 2012 Oct 2.Fig 2.; October 2, 2012 PLoS ONE 7(10): e46666.
Immunohistochemical analysis of rat spinal cord dorsal horn tissue, staining Syntaxin 1a with ab41453 at 1/100 dilution. Fluorescent-conjugated anti-rabbit IgG (1/200) was used as the secondary antibody.
Syntaxin 1a was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to Syntaxin 1a and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab41453. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Band: 33kDa, non specific band - 25kDa: We are unsure as to the identity of this extra band; Syntaxin 1a
Takahashi N et al. Two-photon fluorescence lifetime imaging of primed SNARE complexes in presynaptic terminals and ß cells. Nat Commun6:8531 (2015).
Read more (PubMed: 26439845) »
Zhou J et al. Quantitative Proteomic Analysis Reveals Molecular Adaptations in the Hippocampal Synaptic Active Zone of Chronic Mild Stress-Unsusceptible Rats. Int J Neuropsychopharmacol19:N/A (2015).
Read more (PubMed: 26364272) »