Overview

  • Product name
  • Description
    Rabbit polyclonal to Syntaxin 1a
  • Tested applications
    Suitable for: IHC-FoFr, IP, ICC/IF, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow, Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Syntaxin.

    (Peptide available as ab41452.) On the basis of sequence homology, ab41453 is predicted to react with syntaxin 1B from a number of species, including rat, mouse, human, sheep, cow, zebrafish and chicken.

  • Positive control
    • ab41453 gave a positive signal in the following tissue lysates: Brain (Human); Brain (Mouse); Brain (Rat) ab41453 also gave a positive signal in PC12 (Rat adrenal pheochromocytoma cell line) Cytoplasmic Lysate (data not shown)

Properties

Applications

Our Abpromise guarantee covers the use of ab41453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/3000.
IP Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt 1/300.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).

Target

  • Function
    Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
  • Tissue specificity
    Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.
  • Involvement in disease
    Note=STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • Sequence similarities
    Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • Cellular localization
    Secreted and Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • HPC 1 antibody
    • Neuron specific antigen HPC1 antibody
    • Neuron-specific antigen HPC-1 antibody
    • OTTHUMP00000174615 antibody
    • OTTHUMP00000174616 antibody
    • OTTHUMP00000174617 antibody
    • OTTHUMP00000174618 antibody
    • P35-1 antibody
    • STX1 antibody
    • STX1A antibody
    • STX1A_HUMAN antibody
    • SYN1A antibody
    • Syntaxin 1A (brain) antibody
    • Syntaxin 1A brain antibody
    • Syntaxin-1A antibody
    see all

Anti-Syntaxin 1a antibody images

  • All lanes : Anti-Syntaxin 1a antibody (ab41453) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Mouse Tissue Lysate
    Lane 3 : Brain (Rat) Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • ICC/IF image of ab41453 stained rat PC12 cells. The cells were methanol fixed (5 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab41453, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • ab41453 staining Syntaxin 1a in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/3000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.

    See Abreview

  • Immunohistochemical analysis of rat spinal cord dorsal horn tissue, staining Syntaxin 1a with ab41453 at 1/100 dilution. Fluorescent-conjugated anti-rabbit IgG (1/200) was used as the secondary antibody.
  • Syntaxin 1a was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to Syntaxin 1a and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab41453.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 33kDa, non specific band - 25kDa: We are unsure as to the identity of this extra band; Syntaxin 1a

References for Anti-Syntaxin 1a antibody (ab41453)

This product has been referenced in:
  • Takahashi N  et al. Two-photon fluorescence lifetime imaging of primed SNARE complexes in presynaptic terminals and ß cells. Nat Commun 6:8531 (2015). ICC/IF ; Mouse . Read more (PubMed: 26439845) »
  • Zhou J  et al. Quantitative Proteomic Analysis Reveals Molecular Adaptations in the Hippocampal Synaptic Active Zone of Chronic Mild Stress-Unsusceptible Rats. Int J Neuropsychopharmacol 19:N/A (2015). Read more (PubMed: 26364272) »

See all 5 Publications for this product

Product Wall

Application
Flow Cytometry
Sample
Human Cell (Differentiated hNSCs)
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Gating Strategy
Undifferentiated Stem Cells (white)
Specification
Differentiated hNSCs
Preparation
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation
Paraformaldehyde
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Verified customer

Submitted Aug 18 2015

Application
Immunoprecipitation
Immuno-precipitation step
Protein A
Sample
Rat Tissue lysate - whole (Hippocampal homogenate P1 fraction)
Specification
Hippocampal homogenate P1 fraction
Total protein in input
63.4 µg
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Abcam user community

Verified customer

Submitted Feb 24 2015

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Non-reduced Denaturing (Extra PAGE ONE 5-20%)
Sample
Chicken Tissue lysate - whole (Brain and DRG)
Specification
Brain and DRG
Blocking step
Blocking one as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
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Submitted Feb 13 2015

Application
Western blot
Loading amount
4 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Sample
Rat Tissue lysate - whole (hippocampus,tissue lysate)
Specification
hippocampus,tissue lysate
Treatment
RIPA
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Username

Mr. Jones liu

Verified customer

Submitted Jun 04 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skeletal muscle)
Specification
Skeletal muscle
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X 100 15 min.
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Apr 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

Submitted Jun 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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