Recombinant
RabMAb

Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889)

Overview

  • Product name
    Anti-Syntaxin 1a antibody [EPR11073(B)]
    See all Syntaxin 1a primary antibodies
  • Description
    Rabbit monoclonal [EPR11073(B)] to Syntaxin 1a
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Syntaxin 1a aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q16623

  • Positive control
    • Human cerebellum and fetal brain lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab170889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 33 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
    • Tissue specificity
      Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.
    • Involvement in disease
      Note=STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
    • Sequence similarities
      Belongs to the syntaxin family.
      Contains 1 t-SNARE coiled-coil homology domain.
    • Cellular localization
      Secreted and Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • HPC 1 antibody
      • Neuron specific antigen HPC1 antibody
      • Neuron-specific antigen HPC-1 antibody
      • OTTHUMP00000174615 antibody
      • OTTHUMP00000174616 antibody
      • OTTHUMP00000174617 antibody
      • OTTHUMP00000174618 antibody
      • P35-1 antibody
      • STX1 antibody
      • STX1A antibody
      • STX1A_HUMAN antibody
      • SYN1A antibody
      • Syntaxin 1A (brain) antibody
      • Syntaxin 1A brain antibody
      • Syntaxin-1A antibody
      see all

    Images

    • Western blot analysis on immunoprecipitation pellet from fetal brain lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170889 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

       

      Lane 1 Primary: ab170889, 1/1000 dilution
      Sample: Immunoprecipitated Human fetal brain lysate
      Secondary: HRP-conjugated anti-rabbit IgG

       

      Lane 2 Primary: ab170889, 1/1000 dilution
      Sample: 1X PBS (negative control)
      Secondary: HRP-conjugated anti-rabbit IgG

       

       

      Predicted band 33 kDa

    • All lanes : Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889) at 1/1000 dilution

      Lane 1 : Human cerebellum lysate
      Lane 2 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 33 kDa

    References

    This product has been referenced in:
    • Gao W  et al. IL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury. Front Neurol 8:281 (2017). Read more (PubMed: 28713327) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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