Anti-Syntaxin 1A + Syntaxin 1B2 antibody (ab39986)

Overview

  • Product name
    Anti-Syntaxin 1A + Syntaxin 1B2 antibody
  • Description
    Goat polyclonal to Syntaxin 1A + Syntaxin 1B2
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Sheep, Chicken, Cow, Human, Zebrafish
  • Immunogen

    Synthetic peptide:

    KDRTQELRTAKD

    , corresponding to N terminal amino acids 2/13 of Human Syntaxin 1A + Syntaxin 1B2

  • Positive control
    • Mouse Brain lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:32,000
    WB: Use at a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 35 kDa. (predicted molecular weight: 33 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Syntaxins are potentially involved in the docking of synaptic vesicles at presynaptic active zones and may play a key role in neurotransmitter exocytosis. Three isoforms are produced by alternative splicing. Isoform 1 is expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain. Syntaxin 1A interacts with VAPA and SYBU and is a member of the SNARE core complex containing SNAP25 and VAMP2. In addition, it is found in complexes with SNAP25, SNAP23 and VAMP8. Haploinsufficiency of STX1A may result in particular cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome, a rare developmental disorder.
    • Cellular localization
      Cell membrane; single-pass type IV membrane protein; Isoform 2 is thought to be secreted
    • Database links
    • Alternative names
      • adipocyte C1Q and collagen domain containing antibody
      • adiponectin antibody
      • adipose most abundant gene transcript 1 antibody
      • HPC 1 antibody
      • p35 1 antibody
      • STX1 antibody
      • STX1A antibody
      • STX1B1 antibody
      • STX1B2 antibody
      see all

    Anti-Syntaxin 1A + Syntaxin 1B2 antibody images

    • Anti-Syntaxin 1A + Syntaxin 1B2 antibody (ab39986) at 0.003 µg/ml (for 1 hour) + Mouse Brain lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 33 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    References for Anti-Syntaxin 1A + Syntaxin 1B2 antibody (ab39986)

    This product has been referenced in:
    • Nishiwaki Y  et al. The BH3-only SNARE BNip1 mediates photoreceptor apoptosis in response to vesicular fusion defects. Dev Cell 25:374-87 (2013). Read more (PubMed: 23725763) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry and your interest in our products. Screening our immunizing antigen against other proteins in BLAST-search (against mammalia), ab39986 seems not to cross-react with syntaxin 3. This antibody has not been tested and charac...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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