• Product name
  • Description
    Rabbit polyclonal to Syntaxin 2
  • Tested applications
    Suitable for: ICC/IF, WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Human, Pig, Xenopus laevis, Drosophila melanogaster, Monkey
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 1-19 of Rat Syntaxin 2.

  • Positive control
    • HeLa Cell Lysate, Rat Brain Tissue Extract, Mouse Brain Tissue Extract.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was purified on a peptide bound immunoaffinity column.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab12369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33.3 kDa). human and 1/1000-1/2000 for other species.
IP Use a concentration of 10 µg/ml.
ICC Use at an assay dependent dilution.


  • Function
    Essential for epithelial morphogenesis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
  • Sequence similarities
    Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Form
    Inhibition of syntaxin-2 and Vamp8 function by overexpression of nonmembrane-anchored mutants caused failure of cytokinesis leading to the formation of binucleated cells. There are 3 isoforms produced by alternative splicing.
  • Alternative names
    • EPIM antibody
    • Epimorphin antibody
    • EPM antibody
    • MGC51014 antibody
    • OTTHUMP00000238636 antibody
    • OTTHUMP00000238637 antibody
    • Stx2 antibody
    • STX2_HUMAN antibody
    • STX2A antibody
    • STX2B antibody
    • STX2C antibody
    • Syntaxin 2A antibody
    • Syntaxin 2B antibody
    • Syntaxin 2C antibody
    • Syntaxin-2 antibody
    see all

Anti-Syntaxin 2 antibody images

  • All lanes : Anti-Syntaxin 2 antibody (ab12369) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : Human skin tissue lysate - total protein (ab30166)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33.3 kDa
    Observed band size : 33.3 kDa
    Additional bands at : 74 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ICC/IF image of ab12369 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12369, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Syntaxin 2 antibody (ab12369)

This product has been referenced in:
  • Roqueta-Rivera M  et al. Deficiency in the omega-3 fatty acid pathway results in failure of acrosome biogenesis in mice. Biol Reprod 85:721-32 (2011). Read more (PubMed: 21653892) »
  • Boström P  et al. SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated in insulin sensitivity. Nat Cell Biol 9:1286-93 (2007). WB ; Mouse . Read more (PubMed: 17922004) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry which has been forwarded to me as my colleague Karin, who you originally spoke to, is not available in the office at the moment. I am sorry to confirm that we do not sell larger vial sizes of ab12369 Anti-Syntaxin 2 antib...

Read More


Sign up