Overview

  • Product name
  • Description
    Rabbit polyclonal to Syntaxin 2
  • Tested applications
    Suitable for: ICC/IF, WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Human, Pig, Xenopus laevis, Drosophila melanogaster, Monkey
  • Immunogen

    Synthetic peptide:

    MRDRLPDLTACRKSDDGDN

    conjugated to KLH, corresponding to amino acids 1-19 of Rat Syntaxin 2.

  • Positive control
    • HeLa Cell Lysate, Rat Brain Tissue Extract, Mouse Brain Tissue Extract.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was purified on a peptide bound immunoaffinity column.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33.3 kDa). human and 1/1000-1/2000 for other species.
IP Use a concentration of 10 µg/ml.
ICC Use at an assay dependent dilution.

Target

  • Function
    Essential for epithelial morphogenesis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
  • Sequence similarities
    Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Form
    Inhibition of syntaxin-2 and Vamp8 function by overexpression of nonmembrane-anchored mutants caused failure of cytokinesis leading to the formation of binucleated cells. There are 3 isoforms produced by alternative splicing.
  • Alternative names
    • EPIM antibody
    • Epimorphin antibody
    • EPM antibody
    • MGC51014 antibody
    • OTTHUMP00000238636 antibody
    • OTTHUMP00000238637 antibody
    • Stx2 antibody
    • STX2_HUMAN antibody
    • STX2A antibody
    • STX2B antibody
    • STX2C antibody
    • Syntaxin 2A antibody
    • Syntaxin 2B antibody
    • Syntaxin 2C antibody
    • Syntaxin-2 antibody
    see all

Images

  • All lanes : Anti-Syntaxin 2 antibody (ab12369) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : Human skin tissue lysate - total protein (ab30166)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33.3 kDa
    Observed band size : 33.3 kDa
    Additional bands at : 74 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ICC/IF image of ab12369 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12369, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Shi L  et al. HIV and HCV Co-Culture Promotes Profibrogenic Gene Expression through an Epimorphin-Mediated ERK Signaling Pathway in Hepatic Stellate Cells. PLoS One 11:e0158386 (2016). WB . Read more (PubMed: 27362846) »
  • Roqueta-Rivera M  et al. Deficiency in the omega-3 fatty acid pathway results in failure of acrosome biogenesis in mice. Biol Reprod 85:721-32 (2011). Read more (PubMed: 21653892) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry which has been forwarded to me as my colleague Karin, who you originally spoke to, is not available in the office at the moment. I am sorry to confirm that we do not sell larger vial sizes of ab12369 Anti-Syntaxin 2 antib...

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