• Product nameAnti-Syntaxin 4 antibody
    See all Syntaxin 4 primary antibodies
  • Description
    Mouse monoclonal to Syntaxin 4
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 19-120 of Human Syntaxin 4



Our Abpromise guarantee covers the use of ab57841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 34 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.


  • FunctionPlasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones.
  • Tissue specificityExpressed in neutrophils and neutrophil-differentiated HL-60 cells. Expression in neutrophils increases with differentiation.
  • Sequence similaritiesBelongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • NY REN 31 antigen antibody
    • p35 2 antibody
    • PLACENTAL antibody
    • Renal carcinoma antigen NY REN 31 antibody
    • Renal carcinoma antigen NY-REN-31 antibody
    • Stx4 antibody
    • STX4_HUMAN antibody
    • STX4A antibody
    • Syntaxin 4 antibody
    • syntaxin 4A (placental) antibody
    • Syntaxin 4A antibody
    • Syntaxin-4 antibody
    see all

Anti-Syntaxin 4 antibody images

  • Predicted band size : 34 kDa
    Syntaxin 4 antibody (ab57841) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab57841 at 10 ug/ml staining Syntaxin 4 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • Overlay histogram showing HL60 cells stained with ab57841 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57841, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Syntaxin 4 antibody (ab57841)

This product has been referenced in:
  • Raychaudhuri S MicroRNAs Overexpressed in Growth-Restricted Rat Skeletal Muscles Regulate the Glucose Transport in Cell Culture Targeting Central TGF-ß Factor SMAD4. PLoS One 7:e34596 (2012). Read more (PubMed: 22506032) »

See 1 Publication for this product

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