Overview

  • Product nameAnti-Syntaxin 6 antibody [3D10]
    See all Syntaxin 6 primary antibodies
  • Description
    Mouse monoclonal [3D10] to Syntaxin 6
  • Tested applicationsSuitable for: Flow Cyt, WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human
  • Immunogen

    Recombinant rat syntaxin 6 fusion protein.

  • EpitopeThe epitope for this antibody has been localized to the amino terminus (residues 1-25) of Syntaxin 6.
  • Positive control
    • Mouse Brain Tissue Extract, rat Brain Tissue Extract.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number3D10
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 30.6 kDa).
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

Anti-Syntaxin 6 antibody [3D10] images

  • ab12370 at 1/200 staining a mouse macrophage cell line by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 24 hours. A FITC conjugated donkey anti-mouse antibody was used as the secondary.

    See Abreview

  • All lanes : Anti-Syntaxin 6 antibody [3D10] (ab12370) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30.6 kDa
    Observed band size : 30.6 kDa
    Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes


  • Predicted band size : 30.6 kDa

    Image from Jonas MC et al, J Cell Sci. 2010 Oct 1;123(Pt 19):3378-88. Epub 2010 Sep 7, Fig 1, doi:10.1242/jcs.068841.

  • Syntaxin 6 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 10µg of Mouse monoclonal to Syntaxin 6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab12370.
    Secondary: Protein G-HRP at 1/500 dilution.
    Band: 25kDa:Syntaxin 6.
  • Overlay histogram showing PC12 cells stained with ab12370 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12370, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Syntaxin 6 antibody [3D10] (ab12370)

This product has been referenced in:
  • Walter AM  et al. The SNARE protein vti1a functions in dense-core vesicle biogenesis. EMBO J N/A:N/A (2014). Read more (PubMed: 24902738) »
  • Cronican AA  et al. Genome-wide alteration of histone H3K9 acetylation pattern in mouse offspring prenatally exposed to arsenic. PLoS One 8:e53478 (2013). WB ; Mouse . Read more (PubMed: 23405071) »

See all 11 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10 %)
Sample Guinea pig Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mrs. Monika Schumacher

Verified customer

Submitted Jan 23 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10 %)
Sample Human Cell lysate - whole cell (Several cell lines)
Specification Several cell lines
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mrs. Monika Schumacher

Verified customer

Submitted Jan 16 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse macrophage cell line)
Specification mouse macrophage cell line
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1.5%
Username

Abcam user community

Verified customer

Submitted Feb 27 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"