Overview

  • Product name
  • Description
    Rabbit polyclonal to Syntaxin 7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human Syntaxin 7 aa 100-150 (internal sequence). The exact sequence is proprietary. NP_003560.2.
    Sequence:

    SLTNFQKVQRQ AAEREKEFVA RVRASSRVSG SFPEDSSKER NLVSWESQTQ


    Database link: O15400

  • Positive control
    • HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab192923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 29 kDa.

Target

Images

  • Detection of Syntaxin 7 by Western Blot of Immunprecipitate.
    Lane 1: ab192923 at 1 µg/ml labeling Syntaxin 7 in 293T whole cell lysate immunoprecipitated using ab192923 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG.
    Detection: Chemiluminescence with exposure time of 30 seconds.

  • All lanes : Anti-Syntaxin 7 antibody (ab192923) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 29 kDa


    Exposure time: 30 seconds


    Lanes 1-3 prepared in RIPA lysis buffer; Lanes 4-5 prepared in NETN lysis buffer.

References

ab192923 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab192923.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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