May be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. Mediates the endocytic trafficking from early endosomes to late endosomes and lysosomes.
Highest expression is found in placenta followed by heart, skeletal muscle, kidney and brain. Low expression is found in pancreas, lung and liver.
Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.
Detection of Syntaxin 7 by Western Blot of Immunprecipitate. Lane 1: ab192923 at 1 µg/ml labeling Syntaxin 7 in 293T whole cell lysate immunoprecipitated using ab192923 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: Control IgG. Detection: Chemiluminescence with exposure time of 30 seconds.
Western blot - Anti-Syntaxin 7 antibody (ab192923)
All lanes : Anti-Syntaxin 7 antibody (ab192923) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate Lane 2 : 293T whole cell lysate Lane 3 : Jurkat whole cell lysate Lane 4 : TCMK1 whole cell lysate Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique
Predicted band size : 29 kDa
Exposure time : 30 seconds
Lanes 1-3 prepared in RIPA lysis buffer; Lanes 4-5 prepared in NETN lysis buffer.