Overview

  • Product name
  • Description
    Rabbit polyclonal to Syntaxin 7
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human Syntaxin 7 aa 100-150 (internal sequence). The exact sequence is proprietary. NP_003560.2.
    Sequence:

    SLTNFQKVQRQ AAEREKEFVA RVRASSRVSG SFPEDSSKER NLVSWESQTQ


    Database link: O15400

  • Positive control
    • HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab192923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 29 kDa.

Target

  • Function
    May be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. Mediates the endocytic trafficking from early endosomes to late endosomes and lysosomes.
  • Tissue specificity
    Highest expression is found in placenta followed by heart, skeletal muscle, kidney and brain. Low expression is found in pancreas, lung and liver.
  • Sequence similarities
    Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • Cellular localization
    Early endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • STX7 antibody
    • STX7_HUMAN antibody
    • Syn7 antibody
    • Syntaxin-7 antibody

Images

  • Detection of Syntaxin 7 by Western Blot of Immunprecipitate.
    Lane 1: ab192923 at 1 µg/ml labeling Syntaxin 7 in 293T whole cell lysate immunoprecipitated using ab192923 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG.
    Detection: Chemiluminescence with exposure time of 30 seconds.

  • All lanes : Anti-Syntaxin 7 antibody (ab192923) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 29 kDa


    Exposure time : 30 seconds

    Lanes 1-3 prepared in RIPA lysis buffer; Lanes 4-5 prepared in NETN lysis buffer.

References

ab192923 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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