Overview

  • Product nameAnti-Syntaxin 8 antibody
    See all Syntaxin 8 primary antibodies
  • Description
    Mouse polyclonal to Syntaxin 8
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human Syntaxin 8 protein (NP_004844.1).

  • Positive control
    • Hela lysate and Syntaxin 8 transfected 293T cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.

Target

  • FunctionVesicle trafficking protein that functions in the early secretory pathway, possibly by mediating retrograde transport from cis-Golgi membranes to the ER.
  • Tissue specificityHighly expressed in heart. Also found in brain, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas.
  • Sequence similaritiesBelongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • Post-translational
    modifications
    Ubiquitinated by HECTD3.
  • Cellular localizationMembrane. Preferentially associated with the early endosome. To lesser extends, also present in late endosome, the plasma membrane and coated pits.
  • Information by UniProt
  • Database links
  • Alternative names
    • CARB antibody
    • STX 8 antibody
    • Stx8 antibody
    • STX8_HUMAN antibody
    • Syntaxin-8 antibody
    • Syntaxin8 antibody
    see all

Anti-Syntaxin 8 antibody images

  • Anti-Syntaxin 8 antibody (ab88308) at 1 µg/ml + Hela cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 27 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Syntaxin 8 antibody (ab88308) at 1 µg/ml

    Lane 1 : Syntaxin 8 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 27 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)

References for Anti-Syntaxin 8 antibody (ab88308)

ab88308 has not yet been referenced specifically in any publications.

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