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Our Abpromise guarantee covers the use of ab38745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 - 6 µg/ml.|
ab38745 staining Syntenin in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized; antigen retrieval was by heat mediation in Tris EDTA buffer, pH 9.0. Samples were incubated with primary antibody (1/50) for 30 minutes at 20°C. An undiluted HRP-conjugated rabbit anti-goat IgG polyclonal was used as the secondary antibody.
ab38745 has not yet been referenced specifically in any publications.