Overview

  • Product nameAnti-Syntenin 2 antibody
    See all Syntenin 2 primary antibodies
  • Description
    Goat polyclonal to Syntenin 2
  • SpecificityThis antibody is expected to recognise both human isoforms (NP_056500; NP_536737).
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Syntenin 2 aa 280-292 (C terminal).
    Sequence:

    LLHHTMDHSIPDA

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 6 µg/ml.

Target

Anti-Syntenin 2 antibody images

  • ab38745 staining Syntenin in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized; antigen retrieval was by heat mediation in Tris EDTA buffer, pH 9.0. Samples were incubated with primary antibody (1/50) for 30 minutes at 20°C. An undiluted HRP-conjugated rabbit anti-goat IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab38745 (4µg/ml) staining of paraffin embedded Human Liver shows staining of cytoplasm in hepatocytes. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

References for Anti-Syntenin 2 antibody (ab38745)

ab38745 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS EDTA Buffer pH 9,0
Sample Human Tissue sections (Colon)
Specification Colon
Permeabilization Yes - Wash Buffer from Dako with Tween
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

Submitted Mar 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"