Anti-Syntenin antibody (ab19903)
Key features and details
- Rabbit polyclonal to Syntenin
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-Syntenin antibody
See all Syntenin primary antibodies -
Description
Rabbit polyclonal to Syntenin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Xenopus laevis, Monkey, Zebrafish -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
Target
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Function
Seems to function as an adapter protein. In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA). May also play a role in vesicular trafficking. Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway. -
Tissue specificity
Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta. -
Sequence similarities
Contains 2 PDZ (DHR) domains. -
Post-translational
modificationsPhosphorylated on tyrosine residues. -
Cellular localization
Cell junction > focal adhesion. Cell junction > adherens junction. Cell membrane. Endoplasmic reticulum membrane. Nucleus. Melanosome. Cytoplasm > cytosol. Cytoplasm > cytoskeleton. Mainly membrane-associated. Localized to adherens junctions, focal adhesions and endoplasmic reticulum. Colocalized with actin stress fibers. Also found in the nucleus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 6386 Human
- Entrez Gene: 53378 Mouse
- Entrez Gene: 83841 Rat
- Entrez Gene: 447442 Xenopus laevis
- Entrez Gene: 325004 Zebrafish
- Omim: 602217 Human
- SwissProt: O00560 Human
- SwissProt: O08992 Mouse
see all -
Alternative names
- MDA-9 antibody
- MDA9 antibody
- Melanoma differentiation-associated protein 9 antibody
see all
Images
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Syntenin knockout HAP1 whole cell lysate (20 µg)
Lane 3: A549 whole cell lysate (20 µg)
Lane 4: HeLa whole cell lysate (20 µg)
Lanes 1-4: Merged signal (red and green). Green - ab19903 observed at 32 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab19903 was shown to recognize Syntenin in wild-type HAP1 cells as signal was lost at the expected MW in Syntenin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Syntenin knockout samples were subjected to SDS-PAGE. ab19903 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
All lanes : Anti-Syntenin antibody (ab19903) at 1 µg/ml
Lane 1 : HEK-293 cell lysate
Lane 2 : Human heart tissue lysate
Lane 3 : HEK-293 cell lysate with Mouse Syntenin peptide (ab20431) at 1 µg/ml
Lane 4 : Human heart tissue lysate with Mouse Syntenin peptide (ab20431) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 32 kDaab19903 detects a band of the expected size (32kDa) in HEK-293 cell lysate. This band is partially quenched by the addition of ab20431. No band is observed in human heart lysate.
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All lanes : Anti-Syntenin antibody (ab19903) at 1 µg/ml
Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate
Lane 3 : Mouse heart lysate with Mouse Syntenin peptide (ab20431) at 1 µg/ml
Lane 4 : Rat heart lysate with Mouse Syntenin peptide (ab20431) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/50000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
ab19903 detects a band of the expected size (32kDa) in both mouse and rat heart lysate. This band is quenched completely by the addition of the immunizing peptide in the mouse lysate and is partially quenched by the addition of ab20431 in the rat heart lysate. -
ICC/IF image of ab19903 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab19903, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (36)
ab19903 has been referenced in 36 publications.
- Ma L et al. Apoptotic extracellular vesicles are metabolized regulators nurturing the skin and hair. Bioact Mater 19:626-641 (2023). PubMed: 35600968
- Njock MS et al. Endothelial extracellular vesicles promote tumour growth by tumour-associated macrophage reprogramming. J Extracell Vesicles 11:e12228 (2022). PubMed: 35656866
- Kang Y et al. Serum and Serum Exosomal CircRNAs hsa_circ_0001492, hsa_circ_0001439, and hsa_circ_0000896 as Diagnostic Biomarkers for Lung Adenocarcinoma. Front Oncol 12:912246 (2022). PubMed: 35747792
- Costa A et al. Investigating the Paracrine Role of Perinatal Derivatives: Human Amniotic Fluid Stem Cell-Extracellular Vesicles Show Promising Transient Potential for Cardiomyocyte Renewal. Front Bioeng Biotechnol 10:902038 (2022). PubMed: 35757808
- Malkin EZ et al. Cell-free DNA topology depends on its subcellular and cellular origins in cancer. JCI Insight 7:N/A (2022). PubMed: 36125881